碧萝芷对辐射所致小鼠各脏器中自由基的清除作用  

Scavenging Effect of Pycnogenol on Free Radicals in Radiation Exposed Mice Organs

作　　者：丁翔[1] 强亦忠[2] 王利利[1] 程跃进[2] 江家贵[2] 崔凤梅[2] 

机构地区：[1]苏州大学附属第一医院,江苏苏州215006 [2]苏州大学医学部放射医学与公共卫生学院,江苏苏州215123

出　　处：《工业卫生与职业病》2011年第5期272-274,共3页Industrial Health and Occupational Diseases

摘　　要：目的观察碧萝芷对60Coγ射线整体照射所致小鼠主要脏器中超氧化物歧化酶(superoxidedis mutase,SOD)活力、丙二醛(malondialdehyde,MDA)含量、抗超氧阴离子自由基能力和抑制羟自由基能力的影响。方法小鼠随机分成3组:正常对照组,辐照对照组及碧萝芷实验组。辐照对照组及碧萝芷实验组各接受1次γ射线照射,同时碧萝芷实验组用碧萝芷灌胃,连续7 d。处死后取肝、脾、肾和睾丸组织,测定SOD活力、MDA含量、抑制羟自由基能力和抗超氧阴离子自由基能力。结果碧萝芷对受照小鼠脏器中SOD活力影响不大,可降低脏器中MDA含量;能增加肝脏、肾脏和睾丸组织中抗超氧阴离子自由基能力和抑制羟自由基能力,增加脾脏抑制羟自由基能力。结论碧萝芷具有一定的抗辐射损伤作用,其通过直接中和超氧阴离子自由基和羟自由基而发挥抗氧化功能,并不增加组织中的抗氧化酶含量。Objective To observe the effects of pycnogenol on contents of SOD and MDA,and the inhibition of superoxide anion and hydroxyl radical in 60Co γ-ray exposed mice organs.Methods The mice were randomly divided into 3 groups i.e.the normal control,irradiation control and pycnogenol experiment groups.The irradiation control and pycnogenol experiment groups were exposed to 60Co γ-ray,and the mice of pycnogenol experiment group were given pycnogenol for 7 days after radiation.The tissues were obtained after being sacrificed,in which the vitality of SOD,the content of MDA and the inhibition of superoxide anion and hydroxyl radical were detected.Results Results showed that after the mice were irradiated pycnogenol had no effect on SOD;however,the content of MDA could be reduced in organs.Pycnogenol could increase the inhibition of superoxide anion and hydroxyl radical in kidney,liver and testicular and could increase the inhibition of hydroxyl radical in spleen.Conclusions Pycnogenol shows certain anti-radiation effect through scavenging the superoxide anion and hydroxyl radical without increasing SOD content.

关 键 词：碧萝芷 Γ射线 自由基 

分 类 号：R14[医药卫生—公共卫生与预防医学]