煤工尘肺及合并肺癌肺组织Bcl-2基因表达及意义  

Expression and Its Significance of Bcl-2 Protein in Lung Tissues of Coal Worker's Pneumoconiosis Complicated with Lung Cancer

作　　者：范雪云[1] 马庆坤[2] 张岩松 马骏 张翔[1] 

机构地区：[1]河北联合大学公共卫生学院,河北唐山063000 [2]唐山钢铁集团职业病防治所,河北唐山063000 [3]国家安全生产监督生产管理局职业医学研究所,北京102300

出　　处：《工业卫生与职业病》2011年第5期286-289,共4页Industrial Health and Occupational Diseases

摘　　要：目的检测煤工尘肺及合并肺癌肺组织Bcl-2基因蛋白表达情况,探讨煤工尘肺与肺癌的关系及其分子学机制。方法收集煤工尘肺及煤工尘肺合并肺癌患者尸解病例为研究对象。依据病理诊断,选择煤工尘肺54例,尘肺合并肺癌10例。选取30例煤工尘肺病变旁正常肺组织(组织结构正常、肺泡间隔无破坏、肺泡腔内未见渗出)作为对照肺组织。利用免疫组化方法检测Bcl-2基因蛋白在肺组织中的表达情况。结果对照肺组织、煤工尘肺肺组织和煤工尘肺合并肺癌肺组织Bcl-2基因蛋白阳性表达率分别为16.7%、24.1%和90.0%,平均光密度值分别为2.08±0.16、2.51±0.19、3.22±0.41。煤工尘肺合并肺癌组阳性表达率高于对照肺组织,差异有统计学意义(2χ=15.96,P<0.05);合并肺癌组肺组织Bcl-2蛋白阳性表达率高于煤工尘肺组,差异有统计学意义(χ2=13.5,P<0.05)。经图像分析软件Image-pro plus 6.0测定,肺癌组Bcl-2蛋白表达量高于对照组(t=12.69,P<0.05)和煤工尘肺组(t=8.54,P<0.05),差异有统计学意义;煤工尘肺组Bcl-2蛋白表达量高于对照组,差异有统计学意义(t=10.42,P<0.05)。Ⅰ、Ⅱ、Ⅲ期煤工尘肺之间肺组织中Bcl-2基因蛋白阳性表达率比较,差异无统计学意义(P>0.05)。结论煤工尘肺合并肺癌肺组织Bcl-2基因蛋白阳性表达率及表达量均明显增高;煤工尘肺肺组织Bcl-2基因蛋白表达量高于正常肺组织。Bcl-2基因蛋白的高表达在煤工尘肺合并肺癌发生中可能起一定作用。Objective To detect the expression of Bcl-2 protein in lung tissues of coal worker＇s pneumoconiosis（CWP）complicated with lung cancer and to explore the relationship between CWP and lung cancer and its molecular mechanism.Methods The dissected lung specimens of CWP were collected.Based on pathologic diagnosis,54 cases of CWP and 10 cases of CWP complicated with lung cancer were selected as the study subject.Since to obtain healthy persons＇ lung tissue was very difficult,the lung tissues of normal structure at the side of pathological tissue from of 30 CWP were selected as the control.Bcl-2 gene proteins for all lung tissues were measured by immunohistochemical Elivision plus method.Chi-Square test and t test were used to analyze the data.Results The positive rates of Bcl-2 protein expression in tissues of the control,CWP and CWP complicated with lung cancer group were 16.7%（5/30）,24.1%（13/54）,and 90.0%（9/10）,respectively.The average values of optical density were 2.08±0.16,2.51±0.19 and 3.22±0.41,respectively.The positive rate of Bcl-2 protein expression in CWP complicated with lung cancer was significantly higher than that of CWP and the control tissue（P0.05）.The quantity of Bcl-2 protein expression in CWP was significantly higher than that of the control tissue（t=10.42,P0.05）.The positive rates of Bcl-2 gene protein expression were 26.8%,24.0% and 12.5% in lung tissue of the Ⅰ,Ⅱand Ⅲ stage CWP respectively.The difference was not significant（P0.05）.Conclusions The expression of Bcl-2 protein distinctively increases in lung tissues of CWP complicated with lung cancer and CWP.The excessive expression of Bcl-2 might play a role in the development lung cancer in CWP.

关 键 词：煤工尘肺 煤工尘肺合并肺癌 BCL-2基因蛋白 免疫组织化学法 

分 类 号：R135.2[医药卫生—劳动卫生]