经前平和经前舒颗粒对愤怒郁怒情绪模型大鼠下丘脑γ氨基丁酸B_2受体表达的影响  

Effect of Jingqianping Granules and Jingqianshu Granules on Expression of Hypothalamic γ-Aminobutyric Acid B2 Receptor in Emotional Rats Models of Anger-out and Anger-in

作　　者：姜英凤[1] 薛玲[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,济南250355

出　　处：《中药新药与临床药理》2011年第5期474-478,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家重点基础研究发展计划(973计划)项目(2011CB505102);国家自然科学基金重点项目(30930110);国家自然科学基金面上项目(30973688);山东省科技发展计划项目(2010GSF10290)

摘　　要：目的探索愤怒、郁怒情绪与吖氨基丁酸B2受体（GABABR2）的关系，以及调肝方药经前平颗粒和经前舒颗粒的中枢干预机制。方法采用居住入侵和社会隔离的方法复制愤怒、郁怒情绪大鼠模型，用RT—PCR和Western Blot的方法检测GABABR2 mRNA和蛋白的表达差异。结果与正常对照组相比，愤怒、郁怒情绪模型大鼠下丘脑GABABR2 mRNA水平和蛋白水平均明显下降（P〈0．01～0．05），而且愤怒模型组的降低程度明显大于郁怒模型组。与各模型组相比，各给药组GABABR2 mRNA水平和蛋白水平均有不同程度的升高。结论大鼠下丘脑GABABR2表达降低可能是影响大鼠愤怒和郁怒情绪的重要共性机制之一；中药经前平和经前舒颗粒对GABABR2表达异常变化具有调节作用。Objective To investigate the expression of γ-aminobutyrie acid B2 receptor （GABABR2）in rats models of anger-out and anger-in, and to explore the intervention mechanism of liver-regulating compound Formulas of Jingqianping Granule and Jingqianshu Granules. Methods The emotional models of anger-out and anger-in were induced in rats by social isolation plus resident-intruder. We analyzed the expression of hypothalamic GABABR2 by Western blot and RT-PCR methods. Results The mRNA and protein expression levels of hypothalamic GABAsR2 in model rats were decreased when compared with that in the normal control group（P 〈 0.05-0.01）, and the decrease of GABABR2 expression in anger-out model group was more significant than that in anger-in model group. Compared with the model groups, mRNA and protein expression levels of hypothalamic GABABR2 were increased in the medication groups to various degrees. Conclusion The decrease of hypothalamic GABABR2 expression is one of common emotional mechanisms in rats with with anger-out and anger-in emotion, and Jingqianping Granules and Jingqianshu Granules can up-regulate the GABABR2 expression level.

关 键 词：经前平颗粒 经前舒颗粒 愤怒情绪 郁怒情绪 下丘脑 γ氨基丁酸B2受体 

分 类 号：R285.5[医药卫生—中药学]