HPLC法测定广藿香油中广藿香酮的含量  

Determination of Pogostone in Patchouli Oil by HPLC

作　　者：易宇阳[1] 陈海明[1] 秦臻[1] 彭绍忠[1] 吴晓丽[1] 苏子仁[1,2] 

机构地区：[1]广州中医药大学新药开发研究中心,广州510006 [2]东莞广州中医药大学中医药数理工程研究院,广东东莞523808

出　　处：《中药新药与临床药理》2011年第5期560-562,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金项目(U0732004);国家"重大新药创制"科技重大专项项目(2009ZX09103-376)

摘　　要：目的建立广藿香油中广藿香酮的高效液相色谱测定法。方法采用高效液相色谱法。色谱柱为YMC—Pack C18色谱柱（250×4．6mm，5μm）；流动相：乙腈-0．2％磷酸溶液（60：40，V／V）；检测波长：310nm；流速：1mL·min-1；柱温：35℃。结果广藿香酮在0．25～5μg范围内呈良好线性关系，r=1．0000，平均回收率为101．16％，RSD=I．49％（n=6）；10批样品中广藿香酮含量在3．54～40．19mg·g-1范围内。结论此法简便快速、准确可靠，可用于广藿香油含量的测定和质量监控。Objective To establish a high performance liquid chromatography （HPLC） method for the determination of pogostone in patchouli oil, and to analyse the quality of 10 batches of patchouli oil. Methods HPLC method was performed with YMC- Pack C18 column （250×4.6 mm, 5 μm） as the chromatographic column. The mobile phase consisted of acetonitrile-0.2 % phosphoric acid （V/V, 60 ： 40） , detected wavelength was 310 nm, the flow rate was 1 mL min-1 and column temperature was set at 35 ℃. Results The linear regression for pogostone was obtained in the range of 0.25-5 μg, r=1.0000, the average recovery was 101.16 % with RSD being 1.49 %（n=6）. The content of pogostone in 10 batches of Patchouli oil range from 3.54 mg g-1 to 40.19 mg g-1. Conclusion The established method is simple and rapid, precise and reliable, can be used for the content determination and quality control of Patchouli oil.

关 键 词：广藿香酮 广藿香油 HPLC 

分 类 号：R284.1[医药卫生—中药学]