眼针对大鼠脑缺血再灌注损伤72h后脑皮质BDNF表达的影响  

The Effect of Eye Acupuncture on Brain-deprived Neurotrophic Factor Expression in Rat cerebral cortex with Ischemia-reperfusion injury for 72 hours

作　　者：王哲[1] 高原[1] 马贤德[1] 赵金茹[1] 井欢[1] 关洪全[1] 王德山[1] 

机构地区：[1]辽宁中医药大学基础医学院,沈阳110032

出　　处：《中国中医基础医学杂志》2011年第9期1007-1009,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家重点基础研究发展计划资助项目(2007CB512702);辽宁省教育厅科学技术研究项目(L2010351)

摘　　要：目的:观察眼针对脑缺血再灌注损伤大鼠脑皮质脑源性神经营养因子(BDNF)表达的影响。方法:线栓法复制脑缺血再灌注损伤大鼠模型,随机分为正常组、假手术组、模型组、眼针组。于再灌注72h后进行大鼠神经功能评分,采用RQ-PCR、免疫组化法、western blot法检测缺血脑皮质BDNFmRNA及蛋白的表达。结果:与模型组比较,眼针组大鼠神经功能评分下降,脑皮质BDNF mRNA的表达和蛋白的表达上升(P<0·01)。结论:眼针能提高脑缺血再灌注损伤大鼠脑皮质BDNF表达水平。Objective：To investigate the effects of eye acupuncture on brain-deprived neurotrophic factor（BDNF） expression and study the mechnism of ischemia-reperfusion injury improved.Methods：Rats established by suture method were randomly divided into the control group,the sham operation group,the model group and the eye-acupuncture group.After reperfusion 72h,the neurophysical behaviours were accessed by ZeaLonga neurophysical impairment marks;the expression of ischemic cerebral cortex BDNF mRNA was measured by RT-PCR method;the expression of ischemic cerebral cortex BDNF protein was detected by immunohisto-chemistry and Western blot technology.Results：After reperfusion 72h,compared with the model group,the neurologic impairment marks of the eye-acupuncture group decreased obviously（P0.05）;The expressions of BDNF mRNA and protein in rat cerebral cortex after the eye acupuncture therapy were obviously decreased（P0.01）.Conclusion：The eye acupuncture therapy can increase the expression of BDNF in rat cerebral cortex with ischemia-reperfusion injury,and protect brain by improving the repair of neurons.

关 键 词：缺血再灌注 脑源性神经营养因子 眼针 脑皮质 

分 类 号：R245.329[医药卫生—针灸推拿学]