健脾化瘀方对裸鼠肝癌新生血管生成的影响  

Influence of Jianpihuayu Recipes of TCM on Angiogenesis of Hepatocellular Carcinoma in Nude Mice

作　　者：钟崇[1] 卢传辉[1] 苏小康[1] 徐发斌[1] 李惠东[1] 

机构地区：[1]广州中医药大学第一附属医院肝胆外科,广东广州510405

出　　处：《中国现代医生》2011年第28期4-5,7,F0003,共4页China Modern Doctor

基　　金：2010年广州中医药大学中医药科研创新项目(10CX019)

摘　　要：目的探讨健脾化瘀方对肝癌裸鼠移植瘤新生血管生成的影响。方法裸鼠人肝癌细胞MHCC97-H模型分成健脾化瘀方治疗组(10只)及对照组(10只)。记录两组肝内移植病灶、肝内转移灶及肺转移灶的数目、瘤体大小、瘤重。用免疫组学方法检测裸鼠人肝癌细胞MHCC97-H模型中肝内移植病灶、肝内转移灶及肺转移灶中CD31、CD34的表达。结果所有裸鼠人肝癌细胞MHCC97-H模型均全部能够成瘤。健脾化瘀方治疗组肝内癌灶体积[(2180±690)mm3]明显低于对照组[(3261±560)mm3](t=11.23,P<0.01)。健脾化瘀方治疗组与对照组相比,裸鼠肝内转移率和肺转移率明显下降(P=0.033,0.011)。健脾化瘀方治疗组与对照组肿瘤组织中CD31指数分别为89.50±22.12和111.1±25.1(P<0.05),CD34指数分别为86.3±32.7和109.2±37.0(P<0.05)。结论健脾化瘀方能抑制肝癌新生血管形成,明显抑制肝癌细胞生长与转移。Objective To investigate the influence of Jianpihuayu recipes of TCM on angiogenesis of hepatocellular carcinoma in nude mice. Methods All 20 human hepatoeellular carcinoma nude mice models （ MHCC97-H ） were randomized to 2 groups ： treatment group and control group. The numbers, size, and weigh of tumor in the liver and lung were observed respectively. The protein level of CD31 and CD34 in 20 liver cancer samples were determined by immunohistochemistry method respectively. Results Metastasis appeared in the two group. The size of intrahepatic tumor [ （ 2180 ± 690 ） mm^3] of treatment group was smaller than that of control group [ （ 3261 ± 560 ） mm^3] （ t=11.23, P 〈 0.01 ）. The rate of intrahepatic and lung metastasis in treatment group decreased significantly than that of control group, respectively （P=0.033,0.011 ）. The index of CD31 and CD34 in treatment group was 89.50 ±22.12 and 86.3 ± 32.7, respectively; while 111.1 ± 25.1 and 109.2± 37.0 in control group （ P 〈 0.05 ）. Conclusion Jianpihuayu recipes of TCM may inhibit the angiogenesis, growth and metastasis of HCC.

关 键 词：肝肿瘤 新生血管生成 裸鼠 免疫组化 健脾化瘀方 

分 类 号：R289.5[医药卫生—方剂学]