超高效液相色谱法快速测定辣椒素类物质  

RAPID DETERMINATION OF CAPSAICINOIDS BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY

作　　者：赵春华 侯倩慧 余季金[3] 

机构地区：[1]青岛华测检测技术有限公司,青岛266101 [2]青岛普仁仪器有限公司,青岛266046 [3]青岛大学,青岛266071

出　　处：《化学分析计量》2011年第5期38-40,共3页Chemical Analysis And Meterage

摘　　要：建立了超高效液相色谱法快速测定辣椒制品中辣椒素、二氢辣椒素、降二氢辣椒素的方法。样品以四氢呋喃-甲醇(体积比1∶1)提取,采用反相高效液相色谱分离。色谱柱为BEH C18,流动相乙腈-水进行梯度洗脱;二极管阵列检测器(DAD),检测波长为280 nm。各成分在1.0～100.0 mL/L范围内线性良好。辣椒素、二氢辣椒素测定结果的相对标准偏差分别为0.8%、1.2%(n=5),回收率分别为93.0%～100.0%、92.9%～97.4%。方法检出限为0.05 mg/mL,定量限为0.25 mg/mL。该法满足辣素等级评价相关标准的要求。A rapid method was developed for determination of capsaicin, dihydroeapsaicin and nordihydroeapsaicin in capsa- icinoids by ultra performance liquid chromatography. The sample was extracted with tetrahydrofuran - methanol （volume ratio was 1 ： 1 ）. The components were separated on a BEH Cls column by gradient elution with acetonitrile - water as the mobile phase, and de- tected with DAD at 280 nm. The linear range was 1.0 - 100.0 mL/L. The RSD were 0, 8 % ,1.2% （n =5） , and the recoveries were 93.0% - 100.0% and 92.9% -97.4% for capsaicin and dihydroeapsaicin, respectively. LOD was 0.05 mg/mL, and LOQ was 0.25 mg/mL. The method meets the requirements of pungency level evaluation on the industrial criterion.

关 键 词：超高效液相色谱 辣椒及辣椒制品 辣椒素同系物 辣味水平 

分 类 号：O657[理学—分析化学]