雪莲组培过程对潮霉素敏感性的研究  

Study on the Sensitivity of Saussurea involucrate to Hygromycin During Tissue Culture

作　　者：唐亚萍[1] 覃建兵[1] 原慧[1] 

机构地区：[1]新疆师范大学分子生物与生物信息研究室,乌鲁木齐830053

出　　处：《种子》2011年第9期11-13,共3页Seed

基　　金：科技部援疆计划项目(200840102-39);新疆师范大学研究生科技创新基金项目(20101211)

摘　　要：以雪莲叶片为外植体,研究了不同浓度的潮霉素对雪莲愈伤组织诱导、生长及分化的影响,结果表明:8.0 mg/L浓度的潮霉素为雪莲叶盘转化筛选的最佳质量浓度,不同浓度(5～50 mg/L)潮霉素对MS+NAA 2.00 mg/L+6-BA 1.00mg/L培养基下雪莲愈伤组织的诱导与生长均有明显的抑制作用,随着潮霉素浓度的升高,毒害程度加深,当潮霉素浓度为20 mg/L时表现为生长的愈伤组织块较小、褐化、甚至死亡。同时,低浓度(0.5～2.0 mg/L)的潮霉素可以提高雪莲愈伤组织的分化率,其中0.5 mg/L潮霉素的质量浓度的诱导培养基内,雪莲愈伤组织的出芽率平均可达98.33%。To study the effects on induction, growth and differentiation of callus （Saussurea involucrate）, the hygromycin and Saussurea involucrate leaves were used as stress and explants, respectively. Cultured on MS ＋ NAA 2.00 mg/L ＋6-BA 1.00 mg/L media added different concentration （5 mg/L to 50 mg/L） of hygromycin, the result showed that hygromycin of 8 mg/L was the best concentration for screening calli, there were significantly inhibition on callus induction and growth, and the damagement increased with the concemtration rise of hygromycin, the callus was greatly damaged and it＇ s characters were small, browning and dying with the concentration of hygromycin up to 20 mg/L. Meanwhile, the low concentration （0.5 mg/L to 2 mg/L） of hygromycin could enhance the rate of callus differentiation, the averge frequcnce of callus germination was up to 98.33% on the medium of callus induction added 0.5 mg/L hygromycin.

关 键 词：雪莲 愈伤组织 潮霉素 

分 类 号：S567.2[农业科学—中草药栽培]