柴胡皂甙-d对人肝癌HepG2细胞周期的阻滞作用及p27表达的影响  

Effect of Saikosaponins-d on cell cycle arrest and p27 expression of HepG2 cells in vitro

作　　者：祝葆华[1] 张国平[1] 李明意[1] 王兰天[1] 苑进凯[1] 

机构地区：[1]广东医学院第一临床学院普外科,湛江524001

出　　处：《中华实验外科杂志》2011年第10期1668-1669,共2页Chinese Journal of Experimental Surgery

基　　金：广东省科技计划资助项目，东莞市科技计划资助项目

摘　　要：目的观察柴胡皂甙-d（SSd）对人肝癌HepG2细胞周期的阻滞作用及对其p27基因表达的影响。方法常规培养人肝癌HepG2细胞至对数生长期，随机分为两组，实验组10mg／L的SSd作用48h，设空白对照；流式细胞术（FCM）检测细胞周期分布；逆转录-聚合酶链反应（RT—PCR）检测细胞p27基因mRNA表达；免疫组织化学SP法检测p27蛋白的表达。结果实验组HepG2细胞G1期百分率为（69．43±3．01）％较对照组（62．83±2．72）％明显增加（P〈0．05），S期细胞百分率为（22．30±0．82）％较对照组（27．23±0．59）％明显减少（P〈0．01）；p27基因mRNA相对表达量（0．566±0．001）较对照组（0．335±0．000）明显增多（P〈0．05）；p27蛋白表达阳性率为（33．9±3．1）％较对照组（21．9±1．7）％亦明显增多（P〈0．01）。结论SSd可导致人肝癌HepG2细胞周期阻滞于G1期，其机制可能与上调p27基因表达有关。Objective To observe the effect of Saikosaponins-d （SSd） on cell cycle arrest and p27 expression of HepG2 cells. Methods HepG2 cells were cultured and divided into two groups randomly. The cell cycle of HepG2 was determined by FCM, the expression level of p27 mRNA and protein was detected by using reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry respectively. Results The proportion of HepG2 cells in G1 phase （ 69.43 ± 3.01 ） % in experimental group was higher than that （ 62. 83 ± 2. 72 ） % in control group significantly （ P 〈 0. 05 ）, but that in S phase （22. 30 ± 0. 82） % was fewer than that （27.23 ± 0. 59） % in control group （ P 〈 0. 01 ）. The p27 mRNA expression level of HepG2 in experimental group （0. 566±0. 001 ） was higher than that （0. 335 ±0. 000） in control group （ P 〈 0. 05 ）, and the p27 protein expression level （ 33.9 ±3.1 ） % was higher than that （ 21.9± 1.7） % of control group （ P 〈 0. 01 ）. Conclusion SSd could up-regulate p27 mRNA and protein expression of HepG2 cells and resulted in cell cycle arrest in GI phase.

关 键 词：癌 肝细胞 柴胡皂甙-D P27 细胞周期 

分 类 号：R285[医药卫生—中药学]