毛节缬草抗抑郁和抗肿瘤活性组分的研究  

Studies of activities component of antidepressie and anticancer on Valeriana of ficinalis L.

作　　者：赵丽辉[1] 张一折[2] 杜娟[1] 韩德明[1] 田坚[1] 孙秀平[1] 

机构地区：[1]长春理工大学国际纳米光子学与生物光子学联合研究中心,吉林长春130022 [2]郑州大学生物工程系,河南郑州450001

出　　处：《东北师大学报（自然科学版）》2011年第3期122-125,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20070902-02)

摘　　要：对毛节缬草乙醇渗漉提取的浓缩物和药材蒸馏获得的水提液中的活性组分进行了分析.分别加水混悬后,通过小鼠体内实验检测了抗抑郁活性组分;醇提浓缩物加水混悬后用氯仿、乙酸乙酯、正丁醇依次萃取、浓缩,各萃取组分通过MTT比色法测定了体外培养的Hella细胞、骨髓瘤细胞的增殖抑制率.实验结果表明:缬草水提取物具有抗抑郁的活性,缬草乙醇提取物乙酸乙酯萃取相、正丁醇萃取相及萃取后水相对细胞增殖抑制作用均不明显;而提取物的氯仿萃取相抗肿瘤作用明显,且表现出一定的剂量相关性.In this report,obtained ethanol concentrate by using percolation,and waterextract by distillation.Add water after suspension,detect antidepressant active component by the mice in vivo.Using chloroform,ethyl acetate,and n-butanol ordinal extraction,ethanol concentrate add water after suspension,get extract and extraction surplus water phase concentrate.Each extraction is determinede on inhibition rate of Hella cells and mylenoma in vitro by MTT colorimetric.The result shows that water extract has antidepressant activity,ethyl acetate extract phase,n-butanol extractphase and surplus water phase of ethanol extractive has not a significant action in inhibition of cell proliferation.The CHCl3 extract phase of ethanol extractive is effective with tumor,and it also shows some of the dose correlation.

关 键 词：缬草 抗肿瘤 抗抑郁 活性组分 

分 类 号：R284[医药卫生—中药学]