法国蜜环菌Armillaria gallica菌株遗传多样性的ISSR分析  

Genetic diversity of Armillaria gallica revealed by ISSR analysis

作　　者：孙立夫[1] 裴克全[2] 张艳华[1] 赵俊[3] 杨国亭[4] 秦国夫[3] 宋玉双[3] 宋瑞清[4] 

机构地区：[1]绍兴文理学院生命科学学院,绍兴312000 [2]中国科学院植物研究所生态中心,北京100093 [3]国家林业局森林病虫害防治总站,沈阳110034 [4]东北林业大学林学院,哈尔滨150040

出　　处：《菌物学报》2011年第5期686-694,共9页Mycosystema

基　　金：浙江省自然科学基金(No.Y3090409);哈尔滨市青年科学基金(No.2002AFQXJ006)

摘　　要：在中国东北地区共采集到53个法国蜜环菌Armillaria gallica菌株,用ISSR(Inter-Simple Sequence Repeat)标记技术对这些菌株进行遗传多样性分析。用6个ISSR引物扩增所得条带表明,ISSR标记在蜜环菌中存在较高的多态性;亲缘关系树状图表明,有3个菌株遗传分化明显;其余50个分别来自3个不同地理居群的菌株聚成一类,亲缘关系较近,没有表现出地理隔离。Fifty-three isolates of Armillaria gallica were collected in the Northeast of China.The genetic diversity of these isolates was revealed by ISSR analysis.Polymorphic bands were generated with 6 primers indicated that high diversity was conserved in the Chinese A.gallica isolates.The dendrogram of genetic relationships demonstrated that 3 isolates of China had generated distinct genetic differentiation.The other 50 isolates which were sampled from 3 different geographic populations were mixed into one cluster,and showed a closer genetic relationship without geographical isolation.

关 键 词：亲缘关系 聚类分析 地理隔离 

分 类 号：Q943[生物学—植物学]