采用EF-1α序列及气生菌丝上的孢子形态特征对九州镰孢菌、拟枝孢镰孢菌和厚垣镰孢菌的鉴定(英文)  

Identification of Fusarium kyushuense,F.sporotrichioides and F.chlamydosporum using EF-1α sequence data and morphological characteristics of conidia in aerial mycelium

作　　者：赵志慧[1] 吕国忠[2] 姜子德[1] 曾莉莎[1] 

机构地区：[1]华南农业大学资源环境学院,广州510642 [2]大连民族学院环境与资源学院,大连116600

出　　处：《菌物学报》2011年第5期713-720,共8页Mycosystema

基　　金：Supported by the National Natural Science Foundation of China(No.30900003);the Basic Research Special Project of the Ministry of Science and Technology of China(No.2006FY120100);the Natural Science Foundation of Guangdong(No.E05202480)

摘　　要：对九州镰孢菌Fusarium kyushuense、厚垣镰孢菌F.chlamydosporum和拟枝孢镰孢菌F.sporotrichioides在气生菌丝上产生的孢子进行了比较。九州镰孢菌在气生菌丝上产生多隔孢子(即中型分生孢子);厚垣镰孢菌在气生菌丝上产生的主要是0隔针叶状分生孢子;拟枝孢镰孢菌在气生菌丝上产生两种类型的分生孢子:芜菁形、单胞分生孢子以及椭圆形、0-1隔的分生孢子。多隔的气生孢子(中型分生孢子)在厚垣镰孢菌和拟枝孢镰孢菌这两个种中偶尔可以观察到,但是不应作为对这两个种进行鉴定的主要特征。EF-1α序列的最小进化树分析表明,来自中国的九州镰孢菌具有独特的单核苷酸多态性,与来自日本的菌株间存在差异。来自中国的厚垣镰孢菌分离株在厚垣镰孢菌复合种(FCSC)中形成独特的菌系。Conidia produced in aerial mycelium of Fusarium kyushuense,F.chlamydosporum and F.sporotrichioides were compared.F.kyushuense produced multi-septate conidia in the aerial mycelium（mesoconidia）.On the contrary,conidia in the aerial mycelium of F.chlamydosporum were mainly 0-septate and spindle-shaped.F.sporotrichioides produced two types of conidia in aerial mycelium：napiform and 0-septate and elliptical and 0-1-septate.Multi-septate aerial conidia（mesoconidia） were occasionally observed in F.chlamydosporum and F.sporotrichioides,and they should not be treated as the key characteristics for their identification.The EF-1α sequence analysis shows that Chinese isolates of F.kyushuense possess unique single nucleotide polymorphisms,distinguishing from Japanese isolates.The isolates of F.chlamydosporum isolated from China shows a separated lineage in F.chlamydosporum species complex（FCSC）.

关 键 词：镰刀菌 中型分生孢子 多芽产孢细胞 复瓶梗 枝孢组 

分 类 号：Q949.32[生物学—植物学]