锦绣杜鹃菌根真菌rDNA ITS序列分析及接种效应研究  

Molecular analysis and inoculation effect of mycorrhizal fungi isolated from hair roots of Rhododendron pulchrum

作　　者：陈真[1,2] 杨兵[1] 张春英[1] 郁书君[2] 罗卿权[1] 

机构地区：[1]上海市园林科学研究所,上海200232 [2]华南农业大学园艺学院,广州510642

出　　处：《菌物学报》2011年第5期729-737,共9页Mycosystema

基　　金：上海市自然科学基金(No.09ZR1429400);国家自然科学基金(No.30972409)

摘　　要：利用rDNA ITS序列对锦绣杜鹃菌根真菌的16个菌株进行了分类分析。根据菌株ITS序列全长计算各菌株间序列相似度和遗传距离,并与GenBank中最相似菌株序列构建系统发育树。结果表明:16个菌株在系统树上聚为3个大分支。其中7个菌株在支持率为100%的基础上与树粉孢属真菌Oidiodendron sp.聚为一类;2个菌株与未鉴定的杜鹃花科植物根系真菌unidentified root associated fungi聚为一类,支持率为100%;其他7个菌株在98%的支持率上与几种未命名的欧石楠类菌根真菌聚为一大类。根据锥虫蓝染色压片检测结果,16个菌株的云锦杜鹃接种苗根系内均观察到菌根侵染细胞,初步确定16个菌株均为杜鹃花类菌根真菌。16个菌株均对云锦杜鹃的生长表现为有益作用,接种幼苗的平均株高和平均干重均高于对照。Sixteen strains of ericoid mycorrhizal endophytes isolated from hair roots of Rhododendron pulchrum were classified with rDNA ITS sequenc.The sequence similarities and genetic distances among the strains were calculated based on the rDNA ITS sequences from 16 strains,and neighbour-joining phylogenetic tree were produced by the ITS sequences of the 16 strains and known ericoid mycorrhizal strains from GenBank.The result showed that 16 strains could be divided into three groups in the phylogenetic tree.Seven strains were identified as Oidiodendron sp.,two strains were clustered with several unidentified root-associated mycorrhizal fungi of Ericaceae plants and the other seven strains were grouped with several unnamed ericoid mycorrhizal fungi.Based on the results of fresh mount of hair roots stained with trypan blue,16 fungal strains were initially identified as ericoid mycorrhizal（ERM） fungi with typical ericoid mycorrhizal coils in epidermal cells of hair roots.All the 16 mycorrhizal strains showed obviously beneficial to growth of the seedlings,and the average height and biomass of the inoculated seedlings were both higher than that of control.

关 键 词：杜鹃花 欧石南类菌根 同源分析 接种 

分 类 号：S685.21[农业科学—观赏园艺]