HPLC-UV法同时测定人血浆中对乙酰氨基酚和双氢可待因的浓度  

Simultaneous Determination of Acetaminophen and Dihydrocodeine Concentrations in Human Plasma by HPLC-UV

作　　者：丁细桃[1] 徐仁爱[2] 邱相君[3] 胡国新[2] 

机构地区：[1]温州医学院机能实验教学中心,温州市325035 [2]温州医学院药学院,温州市325035 [3]河南科技大学医学院,洛阳市471003

出　　处：《中国药房》2011年第38期3588-3590,共3页China Pharmacy

摘　　要：目的:建立同时测定人血浆中对乙酰氨基酚和双氢可待因浓度的方法。方法:血浆经乙酸乙酯、正己烷萃取后,采用高效液相色谱-紫外法进行测定,其中色谱柱为ZorbaxXDB-C18,流动相为乙腈-0.1%三氟乙酸(TFA)-水(12∶40∶48),流速为1.0mL·min-1,检测波长为230nm(0～5.0min)和210nm(5.0～6.2min)。结果:对乙酰氨基酚、双氢可待因血药浓度分别在0.10～20.00mg·L-1(r=0.9997)、2.5～150.0μg·L-1(r=0.9994)范围内线性关系良好,定量下限分别为0.1mg·L-1、2.5μg·L-1;低、中、高3种浓度的相对回收率分别为(100.38±2.19)%、(98.59±1.71)%、(100.30±0.83)%以及(97.52±3.33)%、(97.42±3.13)%、(99.60±2.98)%;日内RSD分别为1.65%～2.24%和2.73%～5.02%,日间RSD分别为1.57%～1.97%和3.80%～5.21%。结论:本方法准确可靠、简便快速,适用于人血浆中对乙酰氨基酚和双氢可待因浓度的同时测定。OBJECTIVE：To develop the method for simultaneous determination of acetaminophen and dihydrocodeine in hu-man plasma.METHODS：The plasma was extracted by ethyl acetate and hexane.HPLC-UV method was adopted.The determina-tion was performed on Zorbax XDB-C 18 with mobile phase consisted of acetonitrile-0.1%trifluoroacetic acid（TFA）-water（12：40：4 8）at the flow rate of 1.0 mL·min-1.The UV detection wavelength was set at 230 nm（0～5.0 min）and 210 nm（5.0～6.2 min）.RESULTS：The liner range was 0.10～20.0 mg·L-1 for acetaminophen（r=0.999 7）and 2.5～150.0μg·L-1 for dihydrocodeine（r=0.999 4）.The determination limit was 0.1 mg·L-1 and 2.5μg·L-1.The intra-day RSD were 1.65%～2.24%and 2.73%～ 5.02%and inter-day RSD were 1.57%～1.97%and 3.80%～5.21%,respectively.The relative recoveries were（100.38±2.19）%,（98.59±1.71）%,（100.30±0.83）%for acetaminophen and（97.52±3.33）%,（97.42±3.13）%,（99.60±2.98）%for dihydroco-deine.CONCLUSION：The method is accurate,reliable,simple and rapid,and it could be used to determine acetaminophen and di-hydrocodeine concentrations in human plasma simultaneously.

关 键 词：高效液相色谱-紫外法 对乙酰氨基酚 双氢可待因 血药浓度 药动学 

分 类 号：R969.3[医药卫生—药理学] R287[医药卫生—药学]