酸洗脱血小板表面HLA-Ⅰ类抗原对血小板活化和凋亡的影响  

Effect of acid eluting HLA-class Ⅰ antigens from platelet surface on platelets activation and apoptosis

作　　者：邹勇[1] 刘相富[1] 刘文达[1] 陆英[1] 覃雪玲[1] 李芳[1] 廖思红[1] 袁青[1] 刘贵莲[1] 林东军[1,2] 

机构地区：[1]中山大学附属第三医院输血科,广东广州510630 [2]中山大学附属第三医院血液科,广东广州510630

出　　处：《中国输血杂志》2011年第8期669-671,共3页Chinese Journal of Blood Transfusion

基　　金：广东省科技计划项目(2008B030301055)

摘　　要：目的探讨酸洗脱血小板表面HLA-Ⅰ类抗原对血小板活化和凋亡的影响。方法将10(人)份健康人机采血小板(0.5 ml/份)用pH3柠檬酸缓冲液处理(同时以PBS处理作对照组)后,利用多色流式细胞技术检测血小板表面HLA-Ⅰ类抗原及P-选择素(CD62p)的表达,Annexin V染色检测血小板的早期凋亡率。结果 pH3柠檬酸缓冲液及PBS处理后的血小板表面HLA-Ⅰ类抗原的表达为(23.83±9.93)%vs.(98.15±2.20)%(P<0.05),CD62p的表达为(45.94±10.13)%vs(20.29±4.24)%(P<0.05),血小板的早期凋亡率为(65.29±15.74)%vs(14.65±5.09)%(P<0.05)。结论 pH3柠檬酸缓冲液处理血小板可有效去除血小板表面HLA-Ⅰ类抗原,并同时诱导血小板的活化和凋亡。Objective To investigate the effect of acid eluting HLA-class I antigens from the surface of platelets on platelet activation and apoptosis. Methods Ten apheresis platelets samples （0. 5 ml/sample） from healthy volunteers were treated by the citric acid solution at pH 3.0（ PBS treatment as control group）. The expression of HLA-class I antigens and P-selectin（CD62P） on platelet surface were analyzed by multicolor flow cytometry. The proportion of early apoptotic platelets was detected by Annexin V staining. Results After treatment with the citric acid solution and PBS,the expression of HLA- class I antigens on platelet surface was （23.83 ±9. 93）% vs （99. 34 ± 1.05）% （P 〈0. 05） ,the expression of CD62P on platelet surface was （45.94 ± 10. 13） % vs （20.29 ± 4. 24 ） % （ P 〈 0.05 ）, the proportion of early apoptotic platelets was （ 65.29 ± 15.74 ） % vs （ 14. 65 ± 5.09 ） % （ P 〈 0. 05 ）. Conclusion Treatment of platelets with the citric acid solution at pH 3.0 can effectively eliminated HLA class - I antigens from platelet surface, but significantly induced platelets activation and apoptosis.

关 键 词：人类白细胞抗原 HLA-Ⅰ类抗体 血小板 柠檬酸 缓冲液 活化 凋亡 

分 类 号：R446.62[医药卫生—诊断学] R457.11[医药卫生—临床医学]