支气管哮喘患者血清TNF-α、IL-6和IL-8水平变化研究  

Study on serum levels of tumor necrosis factor alpha,interleukin-6,interleukin-8 in patients with asthma during actue attact and remission

作　　者：王海燕[1] 贾少丹[2] 赵明[1] 纪霞[2] 

机构地区：[1]山东省青岛市市立医院哮喘病实验室 [2]山东省青岛市市立医院干部保健科

出　　处：《中国误诊学杂志》2011年第27期6564-6565,共2页Chinese Journal of Misdiagnostics

基　　金：青岛市科技局资助项目(No:06-2-2-3-nsh);青岛市外国专家局引进国外技术;管理人才项目(QD2010002)

摘　　要：目的探讨TNF-α、IL-6和IL-8的变化及其在哮喘发病中的意义。方法采用ELISA法检测120例哮喘病急性加重期及治疗后稳定期和80例健康对照者外周血TNF-α、IL-6和IL-8水平,同时测定血清中总IgE水平和第1秒用力呼气容积占预计值百分比(FEV1%)。结果哮喘急性加重期组外周血TNF-α、IL-6和IL-8和IgE水平明显高于治疗后稳定期组和对照组(t=-20.79～17.99,P<0.01)。治疗后稳定期组TNF-α、IL-6和IL-8和IgE亦显著高于对照组(t=-16.21～16.10,P<0.01)。哮喘急性发作期与治疗后稳定期外周血TNF-α、IL-6和IL-8与FEV1%呈负相关(r分别为-0.693、-0.728、-0.533,均P<0.01)。结论 TNF-α、IL-6和IL-8参与了哮喘气道炎症的形成,并与气道阻塞程度密切相关。Objective To study the relationship between the diversity of the state of asthma and the three inflammatory cytokines（TNF-a,IL-6 and 1I.-8）. Methods The levels of TNF-a,IL-6 and 1I.-8 in serum of 120 patients with asthma during acute attack and in remission stage, and 80 healthy controls were measurd by enzyme linked immunosorbent assay （EI.ISA）. While the level of serum IgE was determined and the values of forced expiratory volume in 1 second（FEV; ） were recorded with spirometer. Results The levels of serum TNF-α, I1-6,IL-8 and lgE were higher in acute attack compared with those in remission stage and controls significantly（t=- 20.79- 17.99, P〈 0.01} and also the levels in remission stage were higher than those of controls（t= -16.21-16.10）. Correlation a nalysis showed that the FEV% was negtively correlated to the serum levels of TNF-a, I1-6, II. 8 and IgE{ r =0. 693,0. 728,-0. 533 ;all P〈0.01） during asthma acute attack and in remission stage. Conclusion The results indicate that the three inflammatory cytokines{TNF-a,IL-6 and 1L-8） may be involved in the airway inflammation which results in airflow obstruction.

关 键 词：哮喘/代谢 肿瘤坏死因子a/代谢 白细胞介素6/代谢 白细胞介素8/代谢 

分 类 号：R562.25[医药卫生—呼吸系统]