复方瑞康欣对吗啡依赖SK-N-SH细胞NOS活性和NO浓度的影响  

Influence of Compound Ruikangxin on Activity of Nitric Oxide Synthase and Concentration of Nitric Oxide in Morphine-dependent SK-N-SH Cell Line

作　　者：吴明松[1] 罗素元[1] 李学英[1] 汤贤春[1] 王大忠[1] 

机构地区：[1]遵义医学院,贵州遵义563003

出　　处：《辽宁中医药大学学报》2011年第10期83-85,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：贵州省卫生厅科学技术基金资助项目(D-211)

摘　　要：目的:观察复方瑞康欣对吗啡依赖的SK-N-SH细胞各型NOS活性和NO浓度的影响并探讨其机制。方法:SK-N-SH细胞周期同步化后,用吗啡(10μmol/L)作用48h,然后用复方瑞康欣含药血清干预12h,再用纳洛酮(10μmol/L)作用10min后,检测NOS活性和NO含量。结果:复方瑞康欣含药血清能抑制总NOS和cNOS活性(P<0.01),从而降低NO含量(P<0.01),但对iNOS活性没有影响(P>0.05)。结论:复方瑞康欣的戒毒作用与NOS活性和NO水平有关。Objective：To investigate the effect and mechanism of Compound Ruikangxin on morphine-dependent human neuroblastoma SK-N-SH cell line.Method：After cells being synchronized in free-serum culture medium for 24 hours,and then cultured in medium containing morphine（10μmol/L）for 48 hours,the cells turned into morphine-dependent cells.The morphine-dependent cells were administrated by serum with Compound Ruikangxin for 12 hours and then were precipitated by naloxone.The activity of nitric oxide synthase（NOS）and the concentration of nitric oxide（NO）were measured.Results：The three dosages of Compound Ruikangxin could inhibit the activity of cNOS（P0.01） and decrease concentration of NO（P0.01）.Conclusion：The detoxification effect of Compound Ruikangxin might be linked with the activity of cNOS and the level of NO.

关 键 词：复方瑞康欣 吗啡依赖 一氧化氮 一氧化氮合酶 中药血清药理学 

分 类 号：R962[医药卫生—药理学]