苯二氮艹卓受体在小鼠不同静脉麻醉药遗忘效应中的作用  

Role of benzodiazepine receptor in amnesic effect of different intravenous anesthetics in mice

作　　者：高晗[1] 王委[1] 王露[1] 王娇[1] 翟振国[1] 蔡慧明[1] 戴体俊[1] 

机构地区：[1]徐州医学院麻醉学系,221004

出　　处：《中华麻醉学杂志》2011年第7期802-804,共3页Chinese Journal of Anesthesiology

基　　金：：国家自然科学基金（30872432）

摘　　要：目的评价苯二氮革受体在小鼠异丙酚、依托咪酯和氯胺酮遗忘效应中的作用。方法昆明小鼠288只，雌雄各半，体重18—23g，采用随机数字表法，将其随机分为9组（n=32）：生理盐水＋生理盐水组（NN组）、生理盐水＋脂肪乳组（NF组）、氟马西尼＋生理盐水组（FN组）、生理盐水＋异丙酚组（NP组）、氟马西尼＋异丙酚组（FP组）、生理盐水＋依托咪酯组（NE组）、氟马西尼＋依托咪酯组（FE组）、生理盐水＋氯胺酮组（NK组）和氟马西尼＋氯胺酮组（FK组）。NN组、NF组、NP组、NE组和NK组于训练前10min时腹腔注射生理盐水10ml／kg，于训练前5min时分别腹腔注射生理盐水10ml／kg、脂肪乳10ml／kg、异丙酚25mg／kg、依托咪酯3ms／kg和氯胺酮20mg／kg；FN组、FP组、FE组和FK组于训练前10min时腹腔注射氟马西尼1mg／kg，于训练前5min时分别腹腔注射生理盐水10ml／kg、异丙酚25mg／kg、依托咪酯3mg／kg和氯胺酮20mg／kg。分别采用避暗实验、跳台实验和Morris水迷宫实验测试认知功能。结果与NN组比较，NF组和FN组认知功能各指标比较差异无统计学意义（P〉0．05），NP组跳台实验潜伏期缩短，NE组跳台实验潜伏期缩短，错误次数增多，NK组跳台实验潜伏期缩短，水迷宫实验潜伏期延长（P〈0．05）；与NP组比较，FP组避暗实验潜伏期延长，水迷宫实验潜伏期缩短（P〈0．05）；与NE组比较，FE组避暗实验潜伏期延长，避暗实验和跳台实验错误次数减少（P〈0．05）；与NK组比较，FK组跳台实验潜伏期延长，水迷宫实验潜伏期缩短（P〈0．05）。结论小鼠异丙酚、依托咪酯和氯胺酮的遗忘效应与激活苯二氮革受体有关。Objective To investigate the role of the benzodiazepine receptor in the amnesic effect of propofol, etomidate and ketamine in mice. Methods Two hundred and eighty-eight Kunming mice of both sexes weighing 18-23 g were randomly divided into 9 groups（ n = 32 each） ： group normal saline ＋ normal saline （group NN） ; group normal saline ＋ fat emulsion （group NF） ; group flumazenil ＋ normal saline （group FN） ; group nor- mal saline ＋ propofol （group NP） ; group flumazenil ＋ propofol （group FP） ;group normal saline ＋ etomidate （group NE）; group flumazenil ＋ etcimidate （group FE ）; group normal saline ＋ ketamine （group NK ） and group flumazenil ＋ ketamine （group NK）. Normal saline 10 ml/kg was given IP at 10 min before the tests, and normal saline 10 ml/kg, fat emulsion 10 ml/kg, propofol 25 mg/kg, etomidate 3 mg/kg and ketamine 20 mg/kg at 5 min before the tests in groups NN, NF, NP, NE and NK respectively. Flumazenil 1 mg/kg was given IP at 10 rain before the tests, and normal saline 10 ml/kg, fat emulsion 10 ml/kg, propofol 25 mg/kg, etomidate 3 mg/kg and ketamine 20 mg/kg at 5 min before the tests in groups NN, NF, NP, NE and NK respectively. Darkness-avoiding test, platform-mounting test and Morris water maze test were performed to assess the cognition function. The latency of response and number of error were recorded in each test. Results Propofol, etomidate and ketamine signifi-canfly shortened the duration of latency of response in platform-mounting test as compared with group NN. Etomidate also significantly increased the number of error in platform-mounting test as compared with group NN, while ketamine prolonged the duration of latency of response in Morris water maze test as compared with group NN. Flumazcnil significantly counteracted the above action of the 3 intravenous anesthetics. Conclusion Benzodiazepine receptor may play an important role in the amnesic effect induced by propofol, etomidate and ketamine.

关 键 词：受体 GABA-A 遗忘 二异丙酚 依托咪酯 氯胺酮 

分 类 号：R614[医药卫生—麻醉学]