脊髓NF—κB信号通路在大鼠持续性术后痛中的作用  

Role of NF-κB signaling pathway in spinal cord in persistent postoperative pain in rats

作　　者：胡兴国[1] 范素贞[2] 张云翔[1] 尹显和[2] 曾因明[2] 

机构地区：[1]湖南省桃源县人民医院麻醉与危重医学科,415700 [2]徐州医学院江苏省麻醉与镇痛技术重点实验室

出　　处：《中华麻醉学杂志》2011年第7期833-836,共4页Chinese Journal of Anesthesiology

基　　金：湖南省科技计划一般项目（2009SK3018）

摘　　要：目的评价脊髓NF-κB信号通路在大鼠持续性术后痛中的作用。方法选择鞘内置管成功的成年雄性SD大鼠90只，体重200—250g，采用随机数字表法，将其随机分为3组（n=30）：假手术组（S组）、持续性术后痛组（SMIR组）和吡咯烷二硫代氨基甲酸盐组（PDTC组）。SMIR组和PDTC组采用皮肤／肌肉切口牵拉法制备大鼠持续性术后痛模型；S组仅分离暴露肌肉，未牵拉。术后1d时开始，PDTC组于30s内鞘内注射NF-κB抑制剂PDTC10ng，S组和SMIR组鞘内注射等容量生理盐水，1次／d，连续7d。于术前1d（T0）、术后1d（T1）、3d（T2）、7d（T3）、12d（T4）和22d（T5）时，测定机械缩足反应阈值（MWT）；各时点MWT测定结束后，随机取5只大鼠，断头处死后取脊髓组织，采用ELISA法测定TNF-α含量。结果与S组比较，T1-5，时SMIR组MWT降低，T2时PDTC组MWT降低，T3-5时SMIR组和PDTC组脊髓TNF-α含量升高（P〈0．05或0．01）；与SMIR组比较，T2-5，时PDTC组MWT升高，T3,4时脊髓TNF-α含量降低（P〈0．05或0．01）。结论脊髓NF-κB信号通路参与了大鼠持续性术后痛的发生发展。Objective To investigate the role of NF-κB signaling pathway in the spinal cord in persistent postoperative pain evoked by skin/muscle incision and retraction （SMIR） in rats. Methods Ninety male SD rats weighing 200-250 g in which intrathecal （IT） catheter was successfully implanted without complication were randomly divided into 3 groups （ n = 30 eaeh）： group sham operation （group S） ; groups SMIR and group pyrrolidine dithiocarbarnate （a NF-κB inhibitor） （group PDTC）. Persistent postoperative pain was evoked by SMIR according to the method described by Flatters in groups SMIR and PDTC. PDTC 10 ng in 10 μL was injected IT over 30 s once a day for 7 consecutive days after operation in group PDTC. Mechanical paw withdrawal threshold to yon Frey filament stimulation （MWT） was measured at 1 d before （To ,baseline） and 1, 3, 7, 12 and 22 d after surgery （T1-5）. Five animals in each group were sacrificed at each time point after MWT measurement and their lumbar segments of the spinal cord were removed for determination of TNF-α content （by ELISA）. Results SMIR significantly decreased MWT after operation at T1-5 and increased TNF-α content in the spinal cord at T3-5. PDTC signifi- cantly attenuated SMIR-induced hyperalgesia and increase in TNF-α content in the spinal cord. Conclusion NF-κB signaling pathway in the spinal cord plays an important role in the development of SMIR-induced persistent postoperative pain in rats.

关 键 词：NF-ΚB 脊髓 疼痛 手术后 

分 类 号：R614[医药卫生—麻醉学]