杭州地区2009-2010年急性腹泻患者中杯状病毒的检测和分型  

Detection and typing of caliciviruses from patients with acute diarrhea in Hangzhou area,2009-2010

作　　者：诸葛小玲[1] 崔大伟[1] 吴英萍[2] 郑书发[1] 靳淼[3] 段招军[3] 余斐[1] 许世佳 陈凌晓[1] 陈瑜[1] 

机构地区：[1]浙江大学医学院附属第一医院检验科,杭州310003 [2]浙江大学医学院附属义乌医院检验科 [3]中国疾病预防控制中心病毒病预防控制所 [4]浙江省平湖市第一人民医院检验科

出　　处：《中华流行病学杂志》2011年第10期1022-1025,共4页Chinese Journal of Epidemiology

摘　　要：目的了解2009--2010年杭州地区急性腹泻患者中杯状病毒基因型别和分子流行病学特征。方法收集2009--2010年杭州地区920例急性腹泻患者的粪便标本和流行病学资料，用多重PCR方法进行杯状病毒检测，测定部分标本的阳性扩增片段基因序列，并对序列进行系统进化分析。结果急性腹泻患者杯状病毒检出率为21．8％（201／920），其中诺如病毒（Nv）GI型25例、GⅡ型170例、札如病毒（SAV）11例，4例为NVI型和Ⅱ型混合感染、1例为NVⅡ型和SAV混合感染。NV基因型别包括：GI-1（3株）、GI-2（1株）、GⅡ-4／2006b变异株（7株）、GⅡ-2（1株）、GⅡ-7（1株）和GⅡ-4／2008变异株（2株）；SAV基凶型别包括：GI-2（5株）、GI-1（4株）和GⅡ-1（1株）。杯状病毒的流行在不同季节、年龄组人群均有发病。结论杯状病毒是2009—2010年引起杭州地区急性腹泻的主要病原之一，其病原具有病毒多样性和基因型别多样性，NVGⅡ-4／2006b变异株或类似株可能是2009--2010年杭州地区流行的优势株。Objective To investigate the molecular-epidemiologic characteristics andgenotypes of human calicivirus （HuCVs） in acute diarrhea patients in Hangzhou from 2009 to 2010. Methods Epidemiologic data and fecal specimens were collected from patients with acute diarrhea. HuCVs of 920 specimens were detected by PCR. PCR products of several positive samples were randomly selected and sequenced. All the sequences were analyzed, phylogenetically. Results 201 HuCVs positive cases were identified from 920 facal specimens （21.8%）. 25 isolates would include norovirus G I -type, G Ⅱ -type for 170 strains and sapovirus for 11 strains. Norovirus G I -type and G Ⅱ -type were detected in four specimens at the same time. Other specimens were mixed infection with norovirus G Ⅱ -type and sapovirus. Genotypes of HuCVs showed that norovirus G I subtypes were G I - 1 （3 strains） and G I -2 （ 1 strain）. Norovirus G Ⅱ subtypes were G Ⅱ -4/2006b variant strains （7 strains）, GII-2 （1 strain）, G Ⅱ-7 （1 strain） and G Ⅱ-4/2008 variant strains （2 strains）; Sapovirus subtypes were G Ⅰ -2 （5 strains） ,G I -1 （4 strains） and G Ⅱ-1 （ 1 strain）. The prevalence rates of HuCVs were different in seasons and age groups. Conclusion HuCVs were one of the major pathogens causing acute diarrhea. Both multiple viruses and genotypes of HuCVs were found in the specimens. G Ⅱ -4/2006b variant and similar strains were identified, probably as the prevalent strains from 2009 to 2010 in Hangzhou, Zhejiang province.

关 键 词：杯状病毒 诺如病毒 札如病毒 基因型别 

分 类 号：R51[医药卫生—内科学]