小麦品种贵农22抗条锈基因的遗传分析及分子标记  

Genetic analysis and molecular mapping for stripe rust resistance gene(s) in wheat cultivar Guinong22

作　　者：李强[1] 贺苗苗[1,2] 董海丽[1] 姚强[1,2] 井金学[1] 王保通[1] 

机构地区：[1]西北农林科技大学旱区作物逆境生物学国家重点实验室,植物保护学院,杨凌712100 [2]青海省农林科学院,西宁810016

出　　处：《植物病理学报》2011年第5期495-501,共7页Acta Phytopathologica Sinica

基　　金：公益性(农业)行业科技专项(200903035);高等学校学科创新引智计划项目(B07049);国家“十一五”科技支撑计划项目(2006BAD08A05);陕西省“13115”科技创新工程重大科技专项(2014ZDKG-08)

摘　　要：贵农22是由簇毛麦、硬粒小麦以及普通小麦杂交选育而成的普通小麦品种,其对我国目前所有已知条锈菌生理小种均表现高度抗病。为了明确其抗条锈性遗传基础,并对抗条锈基因进行分子作图,本研究选用条锈菌重要小种CYR29、CYR30、CYR32、CYR33和Su11-11,对贵农22与条锈病感病品种辉县红或铭贤169杂交F2代、BC1F1代、BC1F2代进行抗锈性遗传分析,并对贵农22控制Su11-11抗病性的1对隐性基因进行SSR标记。结果表明,贵农22至少含有3对抗条锈病基因。利用272株贵农22/铭贤169 BC1 F2群体筛选到2个与贵农22控制Su11-11抗性的隐性基因连锁的SSR标记Xwmc44和Xcfa2147,遗传距离分别为5.1和7.3 cM,并将抗病基因定位于小麦1BL上,暂命名为YrGn22。基因来源、抗病性分析以及分子检测结果表明,YrGn22不同于1BL上的已知小麦抗条锈病基因Yr3、Yr9、Yr21和Yr29,可能是1个新基因。Guinong22,a common wheat cultivar originated from the derivative of Haynaldia villosa and Triticum durum,is highly resistant to all the known Chinese races and pathotypes of Puccinia striformis f.sp.tritici（Pst）.To study genetics and develop molecular markers for stripe rust resistance gene（s）,F2,BC1F1 and BC1F2 progenies derived from crosses Guinong 22/Huixianhong or Ginong 22/Mingxian 169 were inoculated with Chinese Pst races CYR29,CYR30,CYR32,CYR33 and Su11-11 at the seedling stage under controlled greenhouse conditions.One of BC1F2 generation was selected to screen the polymorphic marker linked to the gene conferring resistance to Su11-11.The results indicated that Guinong 22 had at least three stripe rust resis-tance genes.Two polymorphic SSR markers,Xwmc44 and Xcfa2147 were linked to the recessive gene conferring resistance to Su11-11 with genetic distance 5.1 and 7.3 cM,repectively.Based on SSR loci,the gene was located on wheat chromosome 1BL and temporarily designated as YrGn22.Gene origination,resistance and molelcular tests suggested that YrGn22 was different from the known stripe rust resistance genes Yr3,Yr9,Yr21 and Yr29 located on chromosome 1BL,and might be a new gene.

关 键 词：小麦条锈病 抗病基因 遗传分析 分子标记 

分 类 号：S432.21[农业科学—植物病理学]