氯过氧化物酶的硫酸高铈氧化增敏同步荧光光谱法分析  

The analysis of chloroperoxidase by the enhanced synchronous fluorescence spectroscopy based on oxidation of ceric sulfate

作　　者：高金卫[1] 蒋育澄[1,2] 

机构地区：[1]陕西师范大学化学与材料科学学院,西安710062 [2]陕西师范大学大分子科学陕西省重点实验室,西安710062

出　　处：《分析试验室》2011年第10期1-4,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20876094);中央高校基本科研业务费专项基金资助

摘　　要：基于酸性介质中Ce(SO4)2对酶蛋白分子肽链上荧光发色团色氨酸和酪氨酸残基的氧化,通过强化共轭体系效应增敏酶蛋白的分子荧光而建立了一种微量氯过氧化物酶的分析方法;在8.53×10-7～4.90×10-6 mol/L范围内呈良好的线性关系,检出限可达6.83×10-7 mol/L。样品分析结果与基于Soret吸收的紫外光谱分析结果接近,相对标准偏差小于1.0%。An analytic method for trace chloroperoxidase was proposed based on the oxidation of fluorophore tryptophan and tyrosine residue by ceric sulfate in an acidic solution to strengthen the conjugated effect and enhance the molecular fluorescence spectrum of enzyme.This method had a good linear relationship over the concentration of chloroperoxide between 8.53×10-7and 4.90×10-6 mol/L and good sensitivity.The detection limit was as low as 6.83×10-7 mol/L.The concentration of the sample determined by this method was identical to that obtained from the measurement of Soret absorption by UV-vis spectrometry.The relative standard deviation was less than 1.0 %.

关 键 词：氯过氧化物酶 硫酸高铈 荧光光谱法 

分 类 号：O657.3[理学—分析化学]