人雌激素硫酸基转移酶基因在雌激素依赖型子宫内膜腺癌组织中的表达及临床意义  

Expression and significance of hEST gene in estrogen-dependent endometrial adenocarcinoma tissues

作　　者：王敏[1] 张传英[1,2] 史春雪[1] 常爽[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,辽宁沈阳110004 [2]吉林省前卫医院妇产科,辽宁沈阳110004

出　　处：《中国实用妇科与产科杂志》2011年第10期767-770,共4页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：辽宁省教育厅科学研究项目(20060962)

摘　　要：目的探讨人雌激素硫酸基转移酶(hEST)基因与雌激素依赖型子宫内膜腺癌发生、发展关系,为其病因学研究提供有意义的帮助。方法采用Western-Blot及RT-PCR方法分别检测中国医科大学附属盛京医院妇科2005年7月至2007年10月40例子宫内膜腺癌组织、20例正常子宫内膜组织、12例不典型增生子宫内膜组织中hEST蛋白及mRNA表达的相对含量。结果 hEST蛋白及mRNA在正常子宫内膜增殖期、分泌期和萎缩内膜组织中的表达差异显著(P<0.01)。hEST蛋白及mRNA在正常子宫内膜组织、不典型增生子宫内膜组织及子宫内膜腺癌组织中的表达含量逐渐降低,三者比较有差异(P<0.01)。hESTmRNA和蛋白含量与子宫内膜腺癌组织学分级、肌层浸润深度、手术病理分期及淋巴结转移等无关(P>0.05)。子宫内膜腺癌组织中,hESTmRNA和蛋白在ER阳性组织中的含量明显高于ER阴性组织,差异显著(P<0.01)。结论 hEST表达降低或缺失可能与雌激素依赖型子宫内膜腺癌的早期形成有一定的关系,但hEST不能作为子宫内膜腺癌病情程度和预后评价的指标。Objective To research relationship between the occurrence and development of endometrial adenocarcinoma, to provide significant assistance to studies on etiology of endometrial adenocarcinoma. Methods Inspect the protein expression of hEST by Western-Blot test, inspect the expression of the mRNA of the hEST by RT-PCR, comparing the difference in different tissues. Results The content of hEST protein and mRNA expression in normal endometrial prolif- eration, secretory period and shrink endometrial tissue was significantly （ P 〈 0. 01 ）. The content of hEST protein and mRNA expression in normal endometrium was the high, and decreased inatypical hyperplasia and endometrial adenocarci- noma tissues. The difference among the three groups was very marked （ P 〈 0.01 ）. In endometrial adenocarcinoma tis- sues with different clinical stage, pathological, muscular invasion and so on, the expression level of the mRNA and pro- tein of hEST were of no difference（ P 〉 0.05 ）. In endometrial adenocarcinoma, hEST mRNA and protein in the ER-posifive was significantly higher than the ER-negative with significant differences （ P 〈 0. 01 ）. Conclusions hEST may be related with the tumorigenesis of estrogen-dependent endometrial adenocarcinoma, but hEST wasn＇t index for endometrial adenocarcinoma.

关 键 词：子宫内膜腺癌 基因 人雌激素硫酸基转移酶 雌激素代谢 

分 类 号：R714.255[医药卫生—妇产科学]