饲粮硒对种公鸡睾丸中硒含量和硒蛋白酶基因mRNA表达的影响  

Effects of Dietary Selenium on Selenium Content and mRNA Relative Expression Level of Selenium Protease Gene in the Testis of Breeder Cocks

作　　者：赵庆[1] 刘爱巧[2] 王晓霞[3] 张洁[4] 刘亭婷[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]北京华都峪口禽业有限公司,北京101206 [3]首都师范大学,北京100048 [4]北京农业职业技术学院畜牧兽医系,北京102442

出　　处：《动物营养学报》2011年第6期1011-1018,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：北京市科委资助项目(D08060500060802)

摘　　要：本试验旨在研究饲粮硒对海兰褐种公鸡睾丸中硒含量和硒蛋白酶基因mRNA表达的影响。选取224只48周龄海兰褐种公鸡,随机分为7个处理,每个处理4个重复,每个重复8只。将亚硒酸钠(SS)和酵母硒(SY)2种硒源分别以3个硒水平(0.2、0.5和0.8 mg/kg)添加到海兰褐种公鸡基础饲粮中,对照组饲喂基础饲粮,进行为期35 d的饲养试验。结果表明:1)饲粮添加SS和SY均能显著提高种公鸡睾丸中硒含量(P<0.01),随着硒添加水平升高,睾丸硒含量也显著升高(P<0.01),且添加SY的组显著高于添加SS的组(P<0.01)。2)饲粮添加0.5 mg/kg SS及0.5和0.8 mg/kg SY可显著提高血浆谷胱甘肽过氧化物酶(GPx)活性(P<0.05)。3)各组碘化甲腺氨酸脱碘酶1(ID1)基因mRNA相对表达水平差异不显著(P>0.05);各试验组磷脂谷胱甘肽过氧化物酶(GPx4)基因mRNA相对表达水平均显著高于对照组(P<0.01),且0.8 mg/kg SY组显著高于0.2 mg/kg SY组(P<0.01);各试验组硫氧还蛋白还原酶2(TrxR2)基因mRNA相对表达水平均显著高于对照组(P<0.01),且2种硒源的0.8 mg/kg组均显著高于0.2 mg/kg组(P<0.05或P<0.01)。结果提示,饲粮中添加SY可增强海兰褐种公鸡抗氧化功能,添加0.8 mg/kg SY效果较好。This experiment was conducted to study the effects of dietary selenium on selenium content and the mRNA relative expression level of selenium protease gene in the testis of Hy-line breeder cocks.Two hundred and twenty-four 48-week-old Hy-line breeder cocks were selected and randomly divided into 7 groups with 4 replicates per group and 8 Hy-line breeder cocks per replicate.Three levels of senium from different sources（sodium selenite,SS and selenium yeast,SY） were added into the basal diet at 0.2,0.5 and 0.8 mg/kg（by selenium content）,respectively,but no extra selenium was added to the diet in the control group.The experiment lasted for 35 days.The results showed as following： 1） the addition of either SS or SY significantly increased selenium content in testis of breeder cocks（P〈0.01）,and SY had a more effect than SS（P〈0.01）.With an increase of the supplemental selenium level,the selenium content in testis of breeder cocks had a significant increase（P〈0.01）.2） The supplementation of 0.5 mg/kg SS,0.5 and 0.8 mg/kg SY significantly increased glutathione peroxidase（GPx） activity in plasma of breeder cocks（P〈0.05）.3） The differences of mRNA relative expression level of iodothyronine deiodinase 1（ID1） gene among all groups were not significant（P〈0.05）.The mRNA relative expression level of GPx4 gene in supplemental groups was significantly higher than that in the control group（P〈0.01）,and 0.8 mg/kg SY group was significantly higher than 0.2 mg/kg SY group（P〈0.01）.The mRNA relative expression level of thioredoxin reductases 2（TrxR2） in supplemental groups was significantly higher than that in the control group（P〈0.01）,and 0.8 mg/kg group was significantly higher than 0.2 mg/kg group（P〈0.05 or P〈0.01）.These results indicate that supplemental SY can significantly increase the antioxidant capacity in Hy-line breeder cocks,and 0.8 mg/kg SY has a better effect.

关 键 词：硒 公鸡 睾丸 硒蛋白酶基因mRNA 

分 类 号：S816.7[农业科学—饲料科学]