缺铁胁迫下转番茄铁载体蛋白基因八棱海棠对矿质元素吸收的影响  

Effects of the iron-deficiency on divalent metals in transgenic lines of Malus robusta by introducing LeIRT2 gene

作　　者：陈月红[1,2] 陈卫平[3] 张计育[1,4] 章镇[1] 吕东[1] 戴强[1] 渠慎春[1] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省丘陵地区南京农业科学研究所,江苏南京210046 [3]南京农业大学农学院,江苏南京210095 [4]江苏省中国科学院植物研究所,江苏南京210014

出　　处：《南京农业大学学报》2011年第5期43-46,共4页Journal of Nanjing Agricultural University

基　　金：国家863计划项目(2008AA10Z157);江苏省科技支撑计划项目(BE2008316)

摘　　要：在不同浓度缺铁水培条件下,采用电感耦合等离子体原子发射光谱仪(ICP-AES)测定转基因和非转基因植株叶片和根系Ca2+、Mg2+、Ni2+的含量。研究结果表明:在Fe2+浓度为1μmol.L-1时,转番茄铁载体蛋白基因(LeIRT2)八棱海棠株系叶片中Mg2+的含量显著低于非转基因株系,而根系Mg2+的含量显著高于非转基因株系;该浓度下转基因株系根部Ni2+含量显著高于非转基因株系,叶片中Ni2+含量与非转基因株系无显著差异。Fe2+浓度为10μmol.L-1时,转基因株系叶片和根系Mg2+、Ca2+的含量与非转基因株系无显著差异。Fe2+浓度为1μmol.L-1时,转基因株系叶片和根系Ca2+的含量与非转基因株系无显著差异。无铁条件下在叶片和根系中Ca2+、Mg2+含量无显著差异。The transgenic Malus robusta plants with LeIRT2 were selected for further study.The contents of Ca2＋,Mg2＋and Ni2＋ in leaves and roots of the transgenic lines and non-transgenic plants were detected with the method of ICP-AES.The results showed that with the iron concentration of 1 μmol·L-1,the content of Mg2＋ decreased sharply in leaves but increased notably in roots,compared with the non-transgenic plants.At the same concentration,the content of Ni2＋ in roots was significantly higher than that of the compares,while with no obvious difference in leaves.There were no significant difference of the Mg2＋ and Ca2＋ content in leaves or roots between the transgenic and wild plants treated by iron with concentration of 10 μmol·L-1.There were no significant difference of the Ca2＋ content in leaves or roots between the transgenic and wild plants treated by iron with concentration of 1 μmol·L-1.There were no significance differences of Ca2＋,Mg2＋ content in leaves or roots under iron-free conditions.

关 键 词：八棱海棠 LeIRT2基因 缺铁胁迫 

分 类 号：S661.4[农业科学—果树学]