锌和降钙素基因相关肽对仔猪摄食的影响及其机制研究  

Zinc and CGRP on Feed Intake of Piglets: Effects and Mechanism

作　　者：王坤坤[1] 曹崇海[1] 孙建义[1] 刘建新[1] 钱利纯[1] 

机构地区：[1]浙江大学动物科学学院动物分子营养学教育部重点实验室,杭州310029

出　　处：《动物营养学报》2011年第9期1545-1552,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金锌调控仔猪摄食行为的分子机制研究(30771571/C020302)

摘　　要：本试验旨在研究锌和降钙素基因相关肽(CGRP)对仔猪摄食的影响及其机制。试验选用30头9.57 kg左右的"杜×长×大"三元杂交仔猪,随机分为5组(对照Ⅰ组、试验Ⅰ组、对照Ⅱ组、试验Ⅱ组和试验Ⅲ组),每组6头猪。5组均饲喂相同的基础饲粮,饲养试验期14 d。禁食24 h后,对照Ⅰ组、试验Ⅰ组和试验Ⅱ组分别注射0、2和4 mg/kg BW的锌,注射24 h后屠宰;对照Ⅱ组和试验Ⅲ组分别注射0和0.05 mg/kg BW的CGRP,注射2 h后屠宰。结果表明:1)与对照Ⅰ组相比,试验Ⅰ组采食量显著提高(P<0.05),试验Ⅱ组采食量却显著降低(P<0.05);与对照Ⅱ组相比,试验Ⅲ组采食量显著下降(P<0.05)。2)注射锌对仔猪血糖和瘦素水平无显著性影响(P>0.05),而使甘油三酯(TG)、胰岛素和胰高血糖素水平显著降低(P<0.05);注射CGRP显著降低血糖、TG、胰岛素水平(P<0.05),显著提高胰高血糖素水平(P<0.05),而对瘦素水平无显著性影响(P>0.05)。3)与对照Ⅰ组相比,试验Ⅰ组和试验Ⅱ组神经肽Y(NPY)mRNA表达量显著提高(P<0.05),CGRP mRNA表达量和胆囊收缩素(CCK)mRNA表达量显著降低(P<0.05);与对照Ⅱ组相比,试验Ⅲ组NPY mRNA表达量显著降低(P<0.05),CGRP mRNA表达量和CCK mRNA表达量显著提高(P<0.05)。由此可知,一定剂量的锌可通过调节胰岛素和胰高血糖素分泌,诱导食欲神经肽NPY mRNA表达,抑制饱腹神经肽CGRP和CCK mRNA表达,促进仔猪采食。CGRP可通过促进胰高血糖素分泌,抑制食欲神经肽NPY mRNA表达,诱导饱腹神经肽CGRP和CCK mRNA表达,抑制仔猪采食。This experiment was conducted to study the effects of zinc and calcitonin gene-related peptide（CGRP） on feed intake and its regulation mechanism of piglets.Thirty crossed-bred（Duroc×Landrace×Large White） pigs with an average body weight of 9.57 kg were randomly assigned to five groups（control group Ⅰ,trial group Ⅰ,trial groupⅡ,control group Ⅱ and trial group Ⅲ） with 6 piglets per group.Piglets in all the groups were fed with the same basal diet.The feeding trial lasted for 14 days.After 24-hour fasting（free access to water）,piglets in control group Ⅰ,trial group Ⅰ and trial group Ⅱ were injected 0,2 and 4 mg/kg BW zinc,and then slaughtered at 24-hour after injection.Piglets in control group Ⅱ and trial group Ⅲ were injected 0 and 0.05 mg/kg BW CGRP,and then slaughtered at 2-hour after injection.The results showed as follows： 1） compared with the control group Ⅰ,feed intake of trial group Ⅰ and trial group Ⅱ were significantly increased（P0.05） and decreased（P0.05）,respectively.Compared with the control group Ⅱ,feed intake in trial group Ⅲ were significantly decreased（P0.05）.2） There were no significant differences on serum glucose and leptin levels by injection of zinc（P0.05）,but the triglyceride（TG）,insulin and glucagon levels were significantly decreased（P0.05）.Injection of CGRP significantly decreased serum glucose,TG and insulin levels（P0.05） and increased glucagon levels（P0.05）,but had no effect on leptin levels（P0.05）.3） Compared with the control group Ⅰ,the expression levels of NPY mRNA in trial group Ⅰ and trial group Ⅱ were significantly increased（P0.05）,but the CGRP and CCK mRNA expression levels were significantly decreased（P0.05）.Compared with the control group Ⅱ,the CGRP and CCK mRNA expression level were significantly increased（P0.05）,while NPY mRNA expression levels was decreased significantly（P0.05） in trial group Ⅲ.These results indicate that zinc can stimulate intake of piglets th

关 键 词：锌 CGRP 仔猪 采食量 

分 类 号：S828[农业科学—畜牧学]