痘苗病毒弱毒株广9株的免疫原性分析  

Immunogenicity of Attenuated Vaccinia Virus Guang 9 Strain

作　　者：朱蓉[1,2] 黄维金[2] 王佑春[2] 俞永新[1,2] 

机构地区：[1]武汉生物制品研究所,武汉430060 [2]中国食品药品检定研究院,北京100050

出　　处：《中国生物制品学杂志》2011年第9期1072-1074,共3页Chinese Journal of Biologicals

基　　金：国家"十一五"科技重大专项(2009ZX10004-801)

摘　　要：目的分析痘苗病毒弱毒株广9株(Vaccinia virus Guang 9 strain,VG9)对BALB/c小鼠的免疫原性。方法用鸡胚成纤维细胞培养痘苗病毒VG9株和天坛株(Vaccinia virus Tian Tan strain,VTT),离心纯化病毒,经小鼠背部皮内免疫后,每周采血,共4次,采用ELISA法检测小鼠血清中抗痘苗病毒特异性抗体效价;中和试验检测免疫后3周和4周小鼠血清中和抗体效价。结果两株痘苗病毒免疫后1周,小鼠血清中即产生抗痘苗病毒特异性抗体,效价均达1∶4 050;至第3周,抗体效价上升至1∶12 150;第4周时,VG9株抗体效价维持在1∶12 150,而VTT株效价可达1∶36 450。两株病毒诱导的中和抗体水平均在免疫后3周达峰值,且具有交叉中和作用,均对VTT株中和效果更好,VG9株诱导产生中和抗体的能力较VTT株略低。结论 痘苗病毒VG9株与VTT株一样,具有良好的免疫原性,皮内免疫均能刺激小鼠产生良好的抗痘菌病毒特异性抗体和中和抗体。Objective To analyze the immunogenicity of attenuated vaccinia virus Guang 9（VG9） strain.Methods VG9 and Vaccinia virus Tian Tan（VTT） strains were cultured in chick embryo fibroblasts（CEFs）,purified by centrifugation and inoculated i.d.into BALB / c mice.Serum samples were collected once a week for 4 times,and determined for specific antibody titer against vaccinia virus by ELISA.The neutralizing antibody titers in sera 3 and 4 weeks after immunization were determined by neutralization test.Results Specific antibody titers against vaccinia virus in sera of mice were 1 ∶ 4 050 one week after immunization with both the strains and increased to 1 ∶ 12 150 on week 3.However,the antibody titers against VG9 and VTT strains on week 4 after immunization were 1 ∶ 12 150 and 1 ∶ 36 450 respectively.Both the neutralizing antibody levels induced by the two strains reached peak values 3 weeks after immunization,and cross neutralization was observed.The neutralizing effect on VTT strain was superior to that on VG9 strain,while the ability of VG9 strain in inducing neutralizing antibody was slightly lower than that of VTT strain.Conclusion Both VG9 and VTT strains showed good immunogenicity,with which the immunization by i.d.route induced specific antibody and neutralizing antibody against vaccinia virus in mice.

关 键 词：痘苗病毒 免疫原性 酶联免疫吸附测定 中和试验 

分 类 号：R373.9[医药卫生—病原生物学] R392-33[医药卫生—基础医学]