Ghrelin对鸡等级前卵泡发育的影响  

Effect of Ghrelin on Development of Chicken Prehierarchical Follicles

作　　者：林金杏[1] 毛赟杰[1] 米玉玲[1] 张才乔[1] 

机构地区：[1]浙江大学动物科学学院动物医学系动物生理实验室,杭州310029

出　　处：《畜牧兽医学报》2011年第9期1220-1226,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30871843)

摘　　要：旨在研究Ghrelin对产蛋鸡等级前卵泡发育的调节作用。通过RT-PCR方法检测卵泡Ghrelin受体(GH-SR)的表达情况,采用组织学和免疫组化方法探讨Ghrelin和促卵泡素(FSH)单独或联合处理对鸡等级前卵泡形态学的变化以及层粘连蛋白(LN)和缝隙连接蛋白43(Cx43)的标记率。结果表明,GHSR在颗粒层和膜层上均有表达,其表达量随着卵泡的发育分别至大白卵泡(LWF)和小黄卵泡(SYF)达到最高,随后逐渐降低。悬浮培养的卵泡经Ghrelin和FSH处理24h后,LWF和SYF的颗粒层和膜层厚度及颗粒细胞密度均显著增加(P<0.05)。同时,颗粒细胞中LN和Cx43的标记率显著提高(P<0.05),且以Ghrelin与FSH联合处理组最为显著。结果提示,Ghrelin可通过提高LN和Cx43的表达以增强颗粒细胞的连接功能,促进鸡等级前卵泡颗粒细胞的增殖,并可协同FSH调节卵泡的发育。The effect of Ghrelin on development of chicken prehierarchical follicles was evaluated in this study.The expression of growth hormone secretagogue receptor（GHSR） was determined by RT-PCR,and effects of Ghrelin and follicle stimulating hormone（FSH） on morphology of prehierarchical follicles and laminin and connexin43 labeling indexes in granulosa cells were estimated by histological and immunohistochemical methods.The results showed that GHSR mRNA was expressed in both granulosa and theca layers,and the expression level reached the peak in large white follicle（LWF） and small yellow follicle（SYF）,and then decreased thereafter.After treatment with Ghrelin and FSH for 24 h,the thicknesses of granulosa and theca layers and density of granulosa cells were significantly increased in both LWF and SYF in suspension culture（P0.05）.In addition,the labeling indexes of laminin and connexin43 were remarkably elevated in granulosa cells（P0.05）.An additive effect of FSH with ghrelin was demonstrated.In conclusion,the above results indicate that Ghrelin promotes the expression of laminin and connexin43 in granulosa cells to enhance cell to cell interactions,subsequently stimulates the proliferation of granulosa cells,and cooperates with FSH to regulate chicken prehierarchical follicle development.

关 键 词：GHRELIN 促卵泡素 层粘连蛋白 缝隙连接蛋白 卵泡发育 鸡 

分 类 号：S831.1[农业科学—畜牧学] S852.1[农业科学—畜牧兽医]