MC4R和PROP1基因多态性及合并基因型与中国美利奴羊生长性状的关联分析  

Effects of Single and Combined Genotypes of MC4R and PROP1 Genes on Growth Traits in Chinese Merino Sheep

作　　者：曾献存[1] 陈韩英[2] 贾斌[1] 赵宗胜[1] 惠文巧[1] 张文祥[1] 吴洪宾[1] 王尊宝[1] 

机构地区：[1]石河子大学动物科技学院,石河子832003 [2]石河子大学药学院,石河子832003

出　　处：《畜牧兽医学报》2011年第9期1227-1232,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国际科技合作项目(2007DFB30420)

摘　　要：为了探讨MC4R和PROP1基因作为绵羊生长性状候选基因的可能性,寻找与绵羊生长性状相关的分子标记。本研究采用PCR-SSCP方法检测中国美利奴羊和德国美利奴羊MC4R和PROP1基因的多态性,分析了多态位点不同基因型及合并基因型与中国美利奴羊生长性状的关联性。结果表明:MC4R基因MC4R-3位点存在G894C突变,MC4R-4位点3′侧翼区存在A1223G、G1229A和T1307G3个突变位点;PROP1基因在外显子3上存在A2660G突变,导致Thr181Ala改变。关联分析表明,在中国美利奴羊群体中,MC4R-4位点不同基因型个体体斜长和腰角宽差异显著(P<0.05),优良基因型为BB;PROP1基因不同基因型个体尻宽和胸围差异显著(P<0.05),优良基因型为BB;PROP1-MC4R-4优良基因型聚合体BB-BB个体体斜长高于其它合并基因型个体(P<0.05)。结果提示绵羊MC4R和PROP1基因单核苷酸多态性及合并基因型对中国美利奴羊生长性状有一定的影响,可以作为进行绵羊生长性状分子标记辅助选择的候选基因。This study aimed to study the relationship between MC4R and PROP1 genes and sheep growth traits,and search the markers correlated with sheep growth traits.PCR-SSCP method was applied in this study to detect the polymorphisms of MC4R and PROP1 genes in Chinese Merino and Germany Merino sheep populations,and analyze the association of polymorphisms and combined genotypes with growth traits of Chinese Merino sheep.The results showed that one mutation（G894C） at MC4R-3 site,three mutations（A1223G,G1229A and T1307G） in 3′-untranslated region at MC4R-4 site of MC4R gene,and a transition of A2660G caused an amino acid change（A2660G→Thr181Ala） in exon 3 of PROP1 gene were identified.Correlation analysis showed that,in Chinese Merino sheep population,the effects of MC4R-4 genotypes on body length and hip width were significant（P0.05）,the effective genotype was BB;and the effects of PROP1 genotypes on rump width and chest girth were significant（P0.05）,and the effective genotype was BB.The optical combined genotype of PROP1-MC4R-4 was BB-BB,which is more effective on body length than other combined genotypes（P0.05）.These results indicate that the single nucleotide polymorphisms and combined genotypes of MC4R and PROP1 genes have effect on the growth traits of Chinese Merino sheep,it could be a candidate gene for sheep growth traits and directed to the sheep breeding.

关 键 词：绵羊 MC4R基因 PROP1基因 合并基因型 生长性状 

分 类 号：S826.86[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]