O型口蹄疫抗体金标试纸盒检测免疫动物抗体效价与攻毒保护关系  

Study on the Correlation Between Animal Immunization Antibody Titer and Protection Against FMDV Type O Challenge by Colloidal Gold Immunochromatographic Kit for Detection of the FMDV O Serology

作　　者：祁光宇[1,2] 任维维[1,2] 智晓莹[1,2] 刘西兰[2] 张军 王宇 梁仲[1] 蒋韬[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室/农业部畜禽病毒学重点开放实验室,兰州730046 [2]中农威特生物科技股份有限公司,兰州730046

出　　处：《畜牧兽医学报》2011年第9期1277-1283,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：甘肃省科技厅资助;甘肃科技重大技术专项(1002NKDA037)

摘　　要：本研究目的在于用O型口蹄疫金标试纸条研究免疫动物抗体效价与攻毒保护之间的对应关系以及免疫牛抗体消长规律。用金标试纸条检测了口蹄疫O型疫苗质量标准规定的牛、羊和猪免疫和攻毒血清抗体效价及不同免疫程序的牛血清效价。结果显示,当牛、猪和羊免疫血清稀释≥1∶8时,试纸条是阳性结果,表示有99%的被免疫动物属于抗体水平保护范围;被检测血清稀释≤1∶2时,是阴性结果,表示被免疫动物抗体水平属于不保护范围;血清稀释1∶2～1∶8时,是阳性结果,表示有50%的被免疫动物属于抗体水平保护范围。牛在首次免疫8周后加强免疫可以获得良好的保护率和保护时间。通过本试验确定了金标试纸条检测免疫动物抗体效价与攻毒保护之间的关系及牛加强免疫最佳的时间。The aim of this study was to study the correlation between animal immunization antibody titer and protection against FMDV type O challenge,dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine by Colloidal gold immunochromatographic kit for detection the FMDV O serology.Vaccine testing were determined with FMDV type O inactivated vaccine quality standards,the strips were used in detecting antibody titer of immunized cattle,sheep and pigs and dynamic detections of the antibodies in cattle immunized with the FMD type O vaccine were carried out.The results showed that when immune serum dilution of cattle,pigs and sheep was greater than or equal to 1∶8,the test strip shows positive results,then the antibody levels in 99% immunized animals were in the scope of protection;if the serum dilution was less than or equal to 1∶2,the test strip display negative results,then the antibody levels of immune animals were outside the scope of protection;serum dilution was between 1∶2 and 1∶8,the test strip show positive results,then antibody levels in 50% immunized animals are in the scope of protection.In the present study,we determined the correlation between animal immunization antibody titer and protection against FMDV type O challenge.The results showed that the booster-immunization on eighth week post primary immunization was the most favorable procedure.

关 键 词：O型口蹄疫 金标试纸条 抗体效价 攻毒保护 抗体消长 

分 类 号：S852.659.6[农业科学—基础兽医学]