中国部分地区绵羊肺炎支原体的基因多态性分析  

Investigation of Gene Polymorphism on Mycoplasma ovipneumoniae Isolates from Some Areas of China

作　　者：许健[1,2] 储岳峰[1] 赵萍[1] 高鹏程[1] 贺英[1] 剡根强[2] 逯忠新[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点开放实验室甘肃省生物检测工程技术研究中心,兰州730046 [2]石河子大学动物科技学院,石河子832000

出　　处：《畜牧兽医学报》2011年第9期1295-1301,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：甘肃省科技支撑计划项目(1011NKCA054);甘肃省农业生物技术专项(GNSW-2010-09);国家科技基础性工作专项计划(2008FY210200);国家现代肉羊产业技术体系(CARS-39)

摘　　要：为了解绵羊肺炎支原体(Mycoplasma ovipneumoniae,Mo)在我国部分地区的流行病学特征,采用随机扩增多态性DNA(RAPD)及限制性片段长度多态性(RFLP)方法对26株Mo基因多态性进行了研究,并利用NTsys-2.10e软件对获得的多态性图谱进行了聚类分析。结果在相似系数为0.70时,26株Mo可分成6个RAPD群或6个RFLP群;相似系数为0.90时,分成18个RAPD群或18个RFLP群;相似系数为1.00时,分成25个RAPD群或26个RFLP群。结果表明,我国Mo存在高度的基因多态性,这种多态性与地域差异相关,与宿主来源也具有一定的相关性。研究结果为了解我国Mo的分子流行病学特征打下了基础,也为建立有效的Mo诊断方法和疫苗研制提供了参考依据。To study the epidemiologic characteristics of Mycoplasma ovipneumoniae isolates from some areas of China,the random amplified polymorphic DNA（RAPD） and restriction fragment length polymorphism（RFLP） analysis on 26 strains of M.ovipneumoniae were performed and the gene polymorphism was analyzed by the software NTsys-2.10e in this study.The results showed that the 26 strains of M.ovipneumoniae were divided into 6 RAPD groups or 6 RFLP groups when the similarity coefficient was 0.70,18 RAPD groups or 18 RFLP groups when similarity coefficient was 0.90,25 RAPD groups or 26 RFLP groups when similarity coefficient was 1.00,respectively.The results revealed that M.ovipneumoniae has an obvious gene polymorphism among Chinese isolates.The gene polymorphism of M.ovipneumoniae is highly relevant with the geographical distribution of the strains,and is also relevant with the host origin of strains.This work will provide some insights to study the epidemiology of M.ovipneumoniae and contribute to the development of vaccine against M.ovipneumoniae in China.

关 键 词：绵羊肺炎支原体 随机扩增多态性DNA 限制性片段长度多态性 基因多态性 流行病学 

分 类 号：S852.62[农业科学—基础兽医学]