某海兰褐鸡场祖代、父母代及商品代鸡血管瘤型ALV-J的分离与鉴定  

The Isolation and Detection of Haemangiomas Type of ALV-J about Grandparent,Parent and Commercial Layers in a Hyline Brown Chicken Farm

作　　者：倪伟[1,2] 秦立廷[2] 孙洪磊[1] 曲悦[1] 孙美玉[2] 高玉龙[2] 于宏[3] 王笑梅[2] 刘思当[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验/禽传染病研究室,哈尔滨150001 [3]泰安市畜牧兽医局,泰安271018

出　　处：《畜牧兽医学报》2011年第9期1341-1345,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：现代农业产业技术体系建设专项资金资助(nycytx-42-G3-01);兽医生物技术国家重点实验室开放课题基金(SKLVBF201005)

摘　　要：从山东省某海兰褐鸡场祖代、父母代种鸡和商品代蛋鸡中获得疑似血管瘤型禽白血病(Avian leukosis,AL)病料。采用病理剖检、IFA、分子生物学检测,确定为J亚群禽白血病。从祖代、父母代病料中各分离到1株J亚群禽白血病病毒(J subgroup of avian leukosis virus,ALV-J),从商品代蛋鸡中分离到4株ALV-J。根据原型毒株HPRS103设计1对gp85基因引物,获得gp85基因序列。获得的gp85基因序列与各亚群参考毒株序列核苷酸同源性比对,结果显示:分离自商品代蛋鸡的Commercial03株、Commercial04株、Commercial06株和父母代分离株Parent02株位于同一分支,同源性在97.2%～97.9%,与HPRS103株同源性94.7%～95.2%;Commercial05株与祖代分离株Grandparent01株在同一分支,与HPRS103株同源性为98.3%,4株分离自商品代的ALV-J同源性为95.0%～99.9%。表明商品代蛋鸡中的ALV-J可能来自父母代或祖代种鸡的垂直传播,也可能来自于其他来源的水平传播。从同一鸡场祖代、父母代及商品代鸡中分离得到ALV-J,这在我国还是首次。对后续研究其基因突变、致瘤机制等奠定了良好的基础。Some suspected haemangiomas type of avian leukosis samples were got from one Hyline brown chinken farm in Shandong Province.Six subgroup J avian leukosis viruses（ALV-J） were isolated and identified,among those were strains two virus strains were isolated from parent and grandparent breeding chickens respectively,the other four strains from commercial chickens.The gp85 gene of all the six ALV-J strains were amplified by PCR with a pair of specific primers designed according to the HPRS103 strain.The analysis of sequence of gp85 gene by DNAstar software showed that Commercial strains 03,04 and 06 were in the same embranchment with Prent02,the rate of homology was between 97.2% and 97.9%,the rate of homology with HPRS103 was between 94.7% and 95.2%;Commercial 05 strain and Grandparent 01 strain were in the same embranchment,the rate of homology with HPRS103 is 98.3%.The rate among four commercial strains is 95.0%-99.9%.All above implied that ALV-J infected in egg-type groups partially comes from grandparent breeding chickens or parent breeding chickens through vertical transmission,or from other resources through horizon transmission.The isolation of the six strains from grandparent,patent and commercial layers is the first reported in China,and it lays a good foundation for the research about gene mutation and tumarigenic mechanism.

关 键 词：血管瘤 J亚型禽白血病 分离鉴定 遗传分析 

分 类 号：S852.659.3[农业科学—基础兽医学]