黑穗醋栗品种亲缘关系的ISSR分析  

Genetic Relationship of Blackcurrant Cultivars Revealed by ISSR Markers

作　　者：宋杨[1] 张春雨[2] 张志东[1] 温景辉[3] 李亚东[1] 吴林[1] 刘海广[1] 

机构地区：[1]吉林农业大学园艺学院,长春130118 [2]吉林大学植物科学学院,长春130062 [3]吉林省农业科学院,长春130033

出　　处：《园艺学报》2011年第9期1747-1752,共6页Acta Horticulturae Sinica

基　　金：农业部公益性行业(农业)科研专项(201103037;200903008-07-07);吉林省科技发展计划项目(20080713)

摘　　要：采用ISSR标记技术对39个黑穗醋栗品种的亲缘关系进行分析。结果表明:13条ISSR引物扩增的总带数(A)从3到11条不等,平均扩增出6.77条带,平均多态性带百分比(P)为64.37%。聚类分析显示,在相似性系数为0.8496处将39个黑穗醋栗品种分为5组。第Ⅰ组包含8个起源国家的品种,说明世界各国之间基因交流较为频繁。‘Ben Lomond’和‘Kantata’同其他品种的遗传距离较远,两者又有诸多优良农艺性状,可作为黑穗醋栗的优良育种材料。The genetic relationship of 39 blackcurrant cultivars was analyzed by inter-simple sequence repeats（ISSR）.The results showed that the total number of bands（A）amplified by 13 ISSR primers ranged from 3 to 11,the average was 6.77,the mean percentage of polymorphic band（P）was 64.37%.Cluster analysis showed that 39 blackcurrant cultivars were divided into 5 groups at 0.8496 level of similarity coefficient.Cultivars in the groupⅠcame from 8 origin countries.It indicated that there was larger gene flow between countries.＇Ben Lomond＇and＇Kantata＇were farther with other blackcurrant cultivars in genetic distances.Both cultivars have high agronomic and fruit quality traits.＇Ben Lomond＇ and＇Kantata＇will be good cultivars in the future breeding programme.

关 键 词：黑穗醋栗 ISSR 品种鉴定 亲缘关系 

分 类 号：S663.9[农业科学—果树学]