马动脉炎病毒RT-LAMP检测方法的建立  

Development of an RT-LAMP assay for the detection of equine arteritis virus

作　　者：黄素文[1,2] 于纪棉[3] 倪健波[2] 王建峰[2] 杨文潮[2] 张超[2] 张吉红[2] 相文华[4] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]宁波出入境检验检疫局,浙江宁波315012 [3]宁波天一职业技术学院,浙江宁波315100 [4]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医科学》2011年第9期892-895,共4页Chinese Veterinary Science

摘　　要：为建立一种快速、简便、特异性高的马动脉炎病毒反转录环介导等温扩增(RT-LAMP)检测方法,针对马动脉炎病毒膜基质蛋白M基因的保守序列设计特异性引物,优化反应条件,建立了马动脉炎病毒的RT-LAMP检测方法并对其特异性和灵敏性进行了检测。结果显示,建立的RT-LAMP检测方法具有良好的特异性,灵敏度为0.12pg,是普通RT-PCR的100倍,检测时间仅需1h,肉眼即可观察检测结果。表明建立的RT-LAMP检测方法可用于进出口马动脉炎病毒的检疫。A reverse transcription loop-mediated isothermal amplification（RT-LAMP） assay was established for rapid,simple and specific detection of equine arteritis virus（EAV） based on a set of primers designed according to the membrane matrix protein M gene sequence of EAV available in the GenBank.The specificity and sensitivity of the RT-LAMP were determined under the optimized amplification condition.The RT-LAMP assay was highly specific and the detection limitation was 0.12 pg,which was about 100 times higher than that of RT-PCR.It can be conducted within 1 h and the detection results were visualized.The rapid,sensitive and specific RT-LAMP assay was therefore a potential useful technique for EAV quarantine.

关 键 词：马动脉炎病毒 膜基质蛋白M基因 反转录环介导等温扩增 检疫 

分 类 号：S852.659.6[农业科学—基础兽医学]