电阻抗式传感器大肠杆菌快速检测方法研究  

Research on electrical impedance biosensor for rapid detection of e.coli bacteria

作　　者：张凤[1,2] 金妍[1,2] 刘德盟[1,2] 葛玉卿[1] 金庆辉[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所传感技术国家重点实验室,上海200050 [2]中国科学院研究生院,北京100039

出　　处：《传感器与微系统》2011年第10期29-32,共4页Transducer and Microsystem Technologies

基　　金：上海市科委科技计划资助项目(09JC1416500;09391911500;10391901600;1052nm06100)

摘　　要：基于电阻抗法原理,完成了细菌快速检测阻抗式传感器的前端设计,并对叉指式电极进行工艺实现;合理设计实验方案,选取奈奎斯特曲线斜率表征细菌数量的变化,完成了大肠杆菌生长过程的阻抗研究;对测量结果进行分析,并设计实验验证,完成了细菌生长过程中奈奎斯特斜率变化的理论分析,其结果与理论推导吻合。该数据分析方法降低了对测试电极和测量环境的要求,缩短了细菌测定所需要的时间,可用于微生物的快速检测中。The front-end design of the impedance-based biosensor for rapid detection of bacterial has been completed based on the impedance microbiology. Proper experimental scheme is designed to research the impedance change during the Escherichia coli growth. The slope of Nyquist plots is chosen to characterize the bacterial quantity. The measurement results is analyzed, and experiments are designed to verify it. The changes are explained in the Nyquist slope during the bacterial growth. And the results are in agreement with the theoretical derivation. The analysis method reduces the requirements of microelectrodes and measuremental environment and shortens the time, which can be used for rapid detection of microorganisms.

关 键 词：阻抗微生物 生物传感器 快速检测 叉指式阵列电极 奈奎斯特曲线斜率 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]