A novel hydrogen peroxide biosensor based on the BPT/AuNPs/graphene/HRP composite  

作　　者：WANG Teng ZHU Yi LI Gai ZHANG ShengYi SONG JiMing MAO ChangJie WU JieYing JIN BaoKang TIAN YuPeng 

机构地区：[1]Key Laboratory of Inorganic Materials Chemistry of Anhui Province, Department of Chemistry, Anhui University, Hefei 230039, China [2]State Key Laboratory of Crystal Materials,Shandong University, Jinan 250100, China

出　　处：《Science China Chemistry》2011年第10期1645-1650,共6页中国科学(化学英文版)

基　　金：supported by the National Natural Science Foundation of China (20875001, 20775001, 20771001, 21071002 & 20905001);the Innovation Team Fund of Anhui Province (2006KJ007TD & KJ2010A030)

摘　　要：A novel hydrogen peroxide biosensor based on the BPT/AuNPs/graphene/HRP composite was developed. Firstly, graphene was prepared under the protection of polyvinylpyrrolidone (PVP), and then the AuNPs/graphene composite was synthesized via in situ decoration. Using biphenyldimethanethiol (BPT) as a connector, the AuNPs/graphene composite was immobilized on the surface of the Au electrode, and whereafter the horseradish peroxidase (HRP) was decorated on the surface of the composite by adsorption. The morphology and structure of the products were characterized by XRD, SEM, TEM and UV-visible spectroscopy. The electrocatalytic performance of the resulting BPT/AuNPs/grapheme/HRP composite (namely, biosensor) was studied by electrochemical instrument. The results show that the biosensor has high sensitivity and fast response to H2O2. In the solution of pH 7.4 with potential -0.2V, the linear response of the biosensor to H2O2 ranges from 5.0×10-6 to 2.5×10-3M with the detection limit of 1.5×10-6M.A novel hydrogen peroxide biosensor based on the BPT/AuNPs/graphene/HRP composite was developed. Firstly, graphene was prepared under the protection of polyvinylpyrrolidone (PVP), and then the AuNPs/graphene composite was synthesized via in situ decoration. Using biphenyldimethanethiol (BPT) as a connector, the AuNPs/graphene composite was immobilized on the surface of the Au electrode, and whereafter the horseradish peroxidase (HRP) was decorated on the surface of the composite by adsorption. The morphology and structure of the products were characterized by XRD, SEM, TEM and UV-visible spectroscopy. The electrocatalytic performance of the resulting BPT/AuNPs/grapheme/HRP composite (namely, biosensor) was studied by electrochemical instrument. The results show that the biosensor has high sensitivity and fast response to H2O2. In the solution of pH 7.4 with potential -0.2V, the linear response of the biosensor to H2O2 ranges from 5.0×10-6 to 2.5×10-3M with the detection limit of 1.5×10-6M.

关 键 词：AuNPs/grapheme BIOSENSOR horseradish peroxidase hydrogen peroxide 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]