神经胶质纤维酸性蛋白在电针治疗大鼠脊髓损伤中的变化及意义  

The Changes and Its Significance of Glial Fibrillary Acidic Protein in Spinal Cord Injury of Rats Treated with Electro-acupuncture

作　　者：李振鹏[1] 孔抗美[1] 邹延澄[1] 郭天明[1] 林耿春[1] 

机构地区：[1]汕头大学医学院第二附属医院骨科,广东汕头515041

出　　处：《汕头大学医学院学报》2011年第3期163-165,F0002,共4页Journal of Shantou University Medical College

基　　金：广东省卫生厅资助课题(A2009445)

摘　　要：目的:探讨神经胶质纤维酸性蛋白(GFAP)在电针治疗大鼠脊髓损伤(SCI)中的变化及意义。方法:以2月龄Wistar大鼠(96只)为受试对象,随机分为对照组(A组)、SCI组(B组)、治疗对照组(C组)和电流刺激治疗组(D组),B、C、D组于T9处行脊髓全横断,D组予电针治疗,用Western blot测定各组损伤段脊髓中GFAP不同时相点表达量及其变化,并以苏木精-伊红和免疫组化染色对受损脊髓进行形态学观察。结果:SCI后GFAP表达显著增多,于损伤后2周达顶峰;经电针治疗,GFAP表达减少。结论:GFAP是SCI后脊髓神经纤维再生的重要阻碍因子,电针治疗对GFAP的表达具有明显的抑制作用。Objective： To study the changes of glial fibrillary acidic protein（GFAP） in spinal cord injury（SCI） of rats treated with electro-acupuncture. Methods： Ninety-six adult Wistar rats were randomlyassigned into control group（A）, SCI group（B）, sham-treatment group（ C）and electroacupuncture treatment group（D）. Group B, C and D were transected spinal cord at the T9 vertebrae level while Group A was just opened the vertebra and their spinal cords were not wounded. Group D was treated with electroacupuncture. Western blot and immunohistoehemical methods were used to examine the change of the expression of GFAP in these tissues. Results： The manifold expression of GFAP was found after SCI, es- pecially at the end of the second week, the expression was inhibited by electroacupuncture. Conclusions： GFAP is a major suppressor factor of spinal cord nerve fiber regeneration after SCI, and the electroacupuncture can obviously inhibit the expression of GFAP.

关 键 词：电针 脊髓损伤 神经胶质纤维酸性蛋白 

分 类 号：R651.2[医药卫生—外科学]