磁性/荧光量子点双功能纳米粒子双标记对大鼠骨髓间充质干细胞生物学特性的影响  

Biological characteristics of rat bone mesenchymal stem cells after magnetic/luminescent quantum dots bifunctional nanoparticles labeling

作　　者：颜荣华[1] 王劲[1] 单鸿[1] 聂立波[2] 张黎明[2] 

机构地区：[1]中山大学附属第三医院放射科分子影像学实验室,510630 [2]中山大学化学与化学工程学院高分子研究所,510275

出　　处：《新医学》2011年第9期574-577,共4页Journal of New Medicine

基　　金：国家自然科学基金委员会-广东省人民政府自然科学联合基金(U1032002);广东省自然科学基金项目(10151008901000188)

摘　　要：目的:利用磁性/荧光量子点双功能纳米粒子双标记大鼠骨髓间充质干细胞(BM-SCs),探讨其对BMSCs生物学特性的影响。方法:分离、纯化及培养大鼠BMSCs。制备二氧化硅(SiO2)包裹的含四氧化三铁(Fe3O4)和碲化镉(CdTe)荧光量子点的磁性及荧光双功能纳米粒子Fe3O4@CdTe@SiO2,利用铁浓度为25 mg/L的Fe3O4@CdTe@SiO2双标记BMSCs,未标记的BMSCs作为对照组。采用细胞计数(CCK-8)试剂盒检测BMSCs细胞毒性和增殖能力,台盼蓝拒染测细胞活力,成骨、成脂诱导检测细胞多向分化能力,评价双标记对BMSCs生物学特性的影响。荧光显微镜和普鲁士蓝染色观察诱导分化后Fe3O4@CdTe@SiO2双标记情况。结果:荧光显微镜下可见双标记组细胞内Fe3O4@CdTe@SiO2纳米粒子显示为红色荧光,荧光和磁性双标记率均达到90%以上。Fe3O4@CdTe@SiO2双标记后细胞存活率为(94±5)%。Fe3O4@CdTe@SiO2对BMSCs无明显细胞毒性,对BMSCs的成脂、成骨分化潜能无明显影响。结论:磁性/荧光量子点双功能纳米粒子(Fe3O4@CdTe@SiO2纳米粒子)双标记对大鼠BMSCs安全有效,对BMSCs的生物学特性无明显影响,有望为MRI和光学成像活体示踪BMSCs提供技术基础。Objective：To evaluate the safety of magnetic and luminescent quantum dots bifunctional nanoparticles dual-labeling in rat bone mesenchymal stem cells（BMSCs）.Methods：Rat BMSCs were isolated,purified,and expanded.Magnetic/luminescent bifunctional nanoparticles（Fe3O4@CdTe@SiO2）,were prepared by simultaneous encapsulation of Fe3O4 and CdTe quantum dots with silicon dioxide（SiO2）.Subsequently,BMSCs were incubated with the Fe3O4@CdTe@SiO2 nanoparticles with iron concentration of 25 mg/L.The viability,cytotoxicity and proliferation activity of dual-labeled BMSCs were evaluated with CCK-8 and trypan blue stain exclusion,respectively.Multilineage differentiation potential of dual-labeled BMSCs was assessed by induced osteoblast and adipocyte differentiation.Intracellular fluorescence and iron were also examined with microscopy and prussian blue staining after induced differentiation.Results：Intracellular red fluorescence dots,representing Fe3O4@CdTe@SiO2 were observed via fluorescent microscopy.Rat BMSCs were effectively dual-labeled with approximately 90% efficiency.Viability and proliferation activity of the Fe3O4@CdTe@SiO2-labeled BMSCs was unaltered,compared with those of the unlabeled cells.Meanwhile,multilineage differentiation capacity was conserved in labeled BMSCs.Conclusion：Fe3O4@CdTe@SiO2 magnetic/luminescent bifunctional nanoparticles are effective and safe for dual-labeling of rat BMSCs,without causing significant effects on the biophysical properties of BMSCs.

关 键 词：间充质干细胞 荧光 量子点 超顺磁性氧化铁 标记 多向分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]