荣昌乳仔猪小肠黏膜二糖酶活性发育规律及影响因素的研究  

Research on the development and influence of disaccharidase activity in the small intestinal mucosa in Rongchang piglets

作　　者：兰云贤[1,2] 杨飞云[1] 汪超[1] 黄金秀[1] 刘作华[1] 

机构地区：[1]重庆市畜牧科学院,重庆402060 [2]西南大学荣昌校区,重庆402060

出　　处：《黑龙江畜牧兽医》2011年第9期1-4,9,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金："十一五"国家科技支撑计划项目(2007BAD51B04);重庆市自然基金重点项目(2008BA1013;2009BA1078)

摘　　要：为了系统研究荣昌乳仔猪二糖酶的发育规律,为荣昌乳仔猪饲养工艺研究及产业化示范优化补料技术提供理论依据,试验选择30窝胎次、产仔数一致的荣昌乳仔猪随机分为补饲(A)组和非补饲(B)组,A组从6日龄开始补饲,B组不补饲,分别在第0,2,5,8,11,15,19,23,28,30,35天取仔猪十二指肠、空肠(前、中、后各段)、回肠测定麦芽糖酶、乳糖酶、蔗糖酶的活性。结果表明:补饲对小肠中3种二糖酶活性有一定的影响,补饲组乳糖酶活性在空肠段8,11,15天显著高于其他各天(P<0.05),蔗糖酶活性在空肠前段和中段11,15天显著高于其他各天(P<0.05);非补饲组麦芽糖酶在十二指肠19天、空肠前段19,15,28天显著高于其他各天(P<0.05),乳糖酶活性在十二指肠15天、空肠前段和中段15,11天显著高于其他各天(P<0.05)。说明乳仔猪进行补饲能提前二糖酶发挥高活性的时间;21日龄断奶二糖酶活性对补饲仔猪的影响小于非补饲组;21日龄断奶仔猪比28日龄断奶仔猪酶活性的波动更大;乳仔猪乳糖酶活性在8,11,15日龄达到较高水平。To provide a theoretical basis for feeding piglets and optimizing supplemental feeding technique with industrialization demonstration,thirty litters of Rongchang piglets with consistent birth order and litter size were randomly divided into two groups.A group was for supplemental feeding within 6 days of age,B group was not for supplemental feeding until age of 35 days.In each group,five segments of small intestine including duodenum,front-jejunum,mid-jejunum,back-jejunum and ileum were collected respectively from 3 piglets on the 0th,2nd,5th,8th,11th,15th,19th,23rd,28th,30th and 35th day,the activities of maltase,lactase and sucrase were determined.The results showed that supplemental feeding had affect on activities of all three disaccharidases compared with the no supplemental feeding.Different ages had no effect on activity of maltase,but obviously increased activity of lactase at ages of 8 days,11 days and 15day in jejunum with supplemental feeding for piglets（P0.05）;at ages of 11 days and 15 days,the sucrase activities were higher than other age in front-jejunum and mid-jejunum（P0.05）.In B group,at age of 19 days,the activity of maltase in duodenum was higher than other ages;at ages of 19 days,15 days,28 days,the activities of maltase in front-jejunum were higher than other ages of days（P0.05）.At age of 15 days in duodenum and front-jejunum,11 days in mid-jejunum,the lactase activities were higher than other ages of days（P0.05）.The results indicate that supplemental feeding for the piglets can move up the phase of higher disaccharidase,reducing the affect of disaccharidase on piglets weaned at an age of 21 days.The piglets weaned at 21 days shows much bigger swings than at 28 days on activities of disaccharidases,the lactase activities for the piglets have reached higher levels at age of 8 days,11 days and 15 days.

关 键 词：二糖酶 荣昌乳仔猪 补饲 断奶 

分 类 号：S811.4[农业科学—畜牧学]