miR-143通过抑制PTN促进人骨髓间充质干细胞成脂分化  

miR-143 Accelerates hMSCs Adipogenesis by Suppressing the Expresssion of PTN

作　　者：伊璨[1,2] 谢伟东[2] 吕青[1,2] 张雅鸥[2] 

机构地区：[1]清华大学生命科学学院,北京100084 [2]清华大学深圳研究生院生命与健康学部,广东深圳518055

出　　处：《现代生物医学进展》2011年第18期3401-3404,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30871428)

摘　　要：目的:研究miR-143调控人骨髓间充质干细胞(hMSCs)成脂分化的新机理。方法:将NC、miR-143、siPTN、miR-143i转入hMSCs中,诱导成脂分化,检测miR-143对成脂分化的影响。经miRNA靶点分析软件Findtar预测出miR-143在人多效生长因子(hPTN)的3'-UTR端有靶点。RT-PCR、western blot研究miR-143与hPTN的关系。构建hPTN 3'-UTR靶位点荧光检测质粒prltk-PTN及其突变质粒prltk-m,验证miR-143是否在人PTN 3'-UTR上有靶点。结果:miR-143促进hMSCs成脂分化,抑制hPTN的mRNA和蛋白表达水平。荧光报告实验证实miR-143在人PTN的3'-UTR上有靶点。结论:miR-143通过与hPTN3'-UTR上的靶点相结合而抑制hPTN的表达,从而促进了hMSCs成脂分化进程。Objective： To investigate the mechanism ofmiR-143 in the regulation of hMSCs adipogenesis. Methods： hMSCs were transfected with NC/miR-143/siPTN/miR-143i and induced into adipogenic differentiation to detect the effect ofmiR-143 on adipogenesis. The miR-143 target in 3＇-UTR of hPTN was predicted by Findtar-soflware of miRNA. The relationship between miR-143 and hPTN was researched by the methods of RT-PCR and western blot. The target of miR-143 predicted in hPTN 3＇-UTR was verified by luciferase expression of prltk-PTN plasmid inserted with hPTN 3＇-UTR segment and prltk-m plasmid of mutant target. Results： miR-143 promoted adipogenic differentiation of hMSCs, and suppressed the expression level of hPTN mRNA and protein. The experiment of luciferase demonstrated that there was miR-143 target in hPTN 3＇-UTR. Conclusion： miR-143 suppressed the expression of hPTN by combining with target in hPTN 3＇-UTR to accelerate the process ofhMSCs adipogenesis.

关 键 词：MIR-143 hPTN 成脂分化 HMSCS 

分 类 号：Q254[生物学—细胞生物学] Q291