金标法与免疫发光法检测CEA在健康体检中的应用  

Application of Detecting CEA by Dot Immunogold Chromatographic Assay and Fluorescence Enzye Immunoassay in Physical Examination

作　　者：郭景玉[1] 张云林[1] 贺先奇[1] 王京秋[1] 何柳[1] 秦婷[1] 

机构地区：[1]解放军316医院检验科,北京100093

出　　处：《现代生物医学进展》2011年第18期3497-3498,共2页Progress in Modern Biomedicine

摘　　要：目的:探讨金标法和免疫发光法检测CEA在健康体检中的应用价值。方法:对728例健康体检的个人同时应用金标法和免疫发光法测定CEA,对结果进行分析。结果:金标法测定的阳性率为0.69%,免疫发光法测定的阳性率为0.41%,两者具有高度一致性。结论:对健康体检人群应先用金标法进行定性,对阳性结果再用免疫发光法进行定量。Objective： To investigate the clinic value of using dot immunogold chromatographic assay and fluorescence enzyme immunoassay to detect CEA in physical examination. Methods： Dot immunogold chromatographic assay and fluorescence enzyme immunoassay were used to detect CEA in 728 patients and the results were compared. Results： There were 5 positive cases （0.69%） in dot immunogold chromatographic assay and 3 positive cases （0.41%） in fluorescence enzyme immunoassay, which showed a high agreement. Conclusion： In the physical examination , CEA should be qualitatively detected by dot immunogold chromatographic assay and then the positive cases were quantitatively detected by dot immunogold chromatographic assay.

关 键 词：金标法 免疫发光法 癌胚抗原 健康体检 

分 类 号：R446[医药卫生—诊断学]