转CrylAC及CrylAC+CpTI基因对棉花上绿盲蝽2种消化酶活性及海藻糖含量的影响  

Effects of Introducing CrylAC and CrylAC+CpTI Genes in Cotton on Two Digestive Enzymes Activities and Trehalose Content of Apolygus lucorum

作　　者：谭永安[1] 柏立新[1] 肖留斌[1] 孙洋[1] 赵洪霞[1] 吴国强[1] 

机构地区：[1]江苏省农业科学院植物保护研究所．江苏南京210014

出　　处：《棉花学报》2011年第5期394-400,共7页Cotton Science

基　　金：现代农业产业技术体系建设专项资金;国家农业行业专项(201103012);国家转基因项目:转基因棉花环境安全评价技术(2008ZX08011-002);国家“十一五”重大科技支撑计划(2006BAD08A07)

摘　　要：以转基因棉国抗22,sGK321及其亲本棉泗棉3号、石远321为材料,研究了上述4种寄主上绿盲蝽淀粉酶、蛋白酶活性及海藻糖含量。结果表明:与亲本棉相比,2种转基因棉绿盲蝽若、成虫淀粉酶、蛋白酶活性和海藻糖含量均值较高,且若虫淀粉酶活性、成虫海藻糖含量在转基因棉国抗22和其亲本棉泗棉3号之间差异显著;同一品种棉花上成虫的淀粉酶、蛋白酶活性和海藻糖含量均高于若虫,并差异极显著。绿盲蝽若、成虫淀粉酶、蛋白酶活性及海藻糖含量在转基因棉及其亲本棉上的频率分布高峰基本一致;但与亲本棉花相比,2种转基因棉所测指标在低区间段分布较少,在高区间段分布较多。The activity of amylase, protease and the content of trehalose in laboratory populations of Apolygus lucorum reared on transgenic cotton cultivars, GK22, sGK321, and their correrponding parents, SM3, SY321, were examined. The activity of amylase, protease and the content of trehalose were greater in the populations on transgenic cotton compared with the parental populations, the activity of amylase for nymph and the content of trehalose were significatatly different between GK22 and SM3. Further more, the activity of amylase, protease and the content of trehalose were significantly greater in adults than nymphs in each population. The peak of frequency was basically the same in populations on the four hosts, but the populations on transgenic cotton cultivars distributed in high-range were more than the parental type populations.

关 键 词：绿盲蝽 棉花 转基因 消化酶 海藻糖 

分 类 号：S562[农业科学—作物学]