超短纤维突变体纤维内源激素及其相关酶在纤维伸长期的变化  

Dynamics of Endogenous Phytohormones and Related Enzymes in Ligon Lintless Mutant Fiber

作　　者：邵明彦[1] 王学德[1] 倪密[1] 程超华[1] 刘英新[1] 袁淑娜[1] 

机构地区：[1]浙江大学农业与生物技术学院农学系,杭州310058

出　　处：《棉花学报》2011年第5期440-446,共7页Cotton Science

基　　金：国家转基因生物新品种培育科技重大专项(2009ZX08009-061B)

摘　　要：以超短纤维突变体(Ligon lintless)及其野生型这一对遗传等基因系为材料,研究棉纤维伸长与内源激素含量及其相关酶活性的关系。结果表明,在野生型纤维快速伸长阶段,突变体却表现为:纤维中GA3初期的低含量、IAA的峰值出现时间推迟(迟6 d)且明显低于野生型、ABA含量的快速降低时期出现迟(迟3 d)且短(短6 d)以及纤维伸长启动期极低含量的ZRs。而突变体纤维中过氧化物酶(POD)和IAA氧化酶含量的高峰均早于野生型,且在纤维快速伸长时期出现;突变体纤维中的色氨酸合成酶活性也低于野生型。推测GA3、IAA和ZRs是纤维伸长的正向影响因子,且IAA作用于整个伸长期,GA3作用于伸长初期,ZRs作用于伸长启动期。而POD、IAA氧化酶和色氨酸合成酶通过调节激素的合成与代谢调控纤维伸长,前两种酶起着抑制作用,后一种酶起着促进作用。An appropriate level of endogenous phytohormones and expression of their relevant enzymes is crucial for normal development of fiber in Gossipium hirsutum L. Ligon lintless mutant with super-short fibers （5-8 mm in length） and its wild type with normal fibers （30 mm in length） were used to study the contents of endogenous phytohormones and related enzymes. ELISA revealed decreased and delayed accumulation of Gibberellic acid （GA3）, Indole-3-acetic acid （IAA） and Zeatin riboside （ZRs） in Ligon lintless mutant as compared to its wild type. Similarly, activity ofindole-3-acetic acid oxidase （IAA oxidase） and peroxidase （POD） was increased significantly whereas activity of tryptophan synthetase was decreased in Ligon lintless mutant as compared to its wild type. These results may suggest that GA3 and ZRs contribute to fiber elongation only at early stages while IAA influenced it throughout fiber elongation phase. Moreover, down regulation oftryptophan synthetase and up regulation of I- AA oxidase and POD mighL influence fiber elongation through regulating synthesis and degradation of endogenous phytohor mones in Ligon lintless mutant.

关 键 词：棉花 纤维伸长 内源激素 酶 超短纤维突变体 

分 类 号：S562[农业科学—作物学]