源库调节对Bt棉棉铃外源杀虫蛋白表达量影响的研究  

Effect of Source-sink Regulation on the Content of Bt Endotoxin in Bt Cotton Boll

作　　者：吉荣龙[1] 崔必波[1] 黄志勇[1] 费月跃[1] 顾克余[1] 孙扣忠[1] 陈德华 

机构地区：[1]江苏沿海地区农科所新洋试验站．江苏盐城224049 [2]扬州大学．江苏扬州225009

出　　处：《棉花学报》2011年第5期454-459,共6页Cotton Science

基　　金：国家自然科学基金(30971727);江苏省重点实验室重大项目(10KJA210057);江苏省青蓝工程和扬州大学新世纪学术带头人基金;江苏省自然科学基金企业博士计划(BK2009653);农业部国家转基因生物新品种培育重大专项(2009ZX08013-014B);农业部国家转基因生物新品种培育重大专项(2009ZX08013-011B)

摘　　要：以转Bt基因抗虫棉不同大小棉铃品种泗抗1号、泗抗3号和科棉6号为材料,2005-2006年于扬州大学实验农场进行源库调节对棉铃Bt杀虫蛋白表达量影响的试验。研究结果表明:整株去除一半叶片,则体积膨大期棉铃体积、铃重和子指变小,且体积和铃重以大铃品种科棉3号受影响大,花后28 d体积和铃重分别下降15.4%和20.5%,子指以小铃品种泗抗1号受影响大,花后28 d下降了20.6%;与体积、铃重结果相反,铃壳和棉子中Bt蛋白含量增加,花后21 d则分别增加了23.5%～32.3%和3.3%～18.8%。整株均匀去除一半蕾,则棉铃体积、铃重和子指变大,其中仍以大铃品种受影响大,花后28 d分别增加了25.8%,10.4%和12.7%;而铃壳和棉子中Bt蛋白含量下降,仍以大铃品种下降幅度大,花后21 d分别下降了40.6%和33.7%。相关分析表明,源库调节下铃体积、铃重都与铃壳和棉子中Bt蛋白含量呈显著负相关,子指与棉子中Bt蛋白也表现同样效应。因此,在育种和栽培时需协调好棉铃发育和Bt表达的关系。The objective of this study was to investigate the change of insecticidal protein content under source-sink regulation in Bt cotton boll. The experiments were conducted at Yangzhou University farm during cotton growth season from 2005 to 2006, three cultivars with different boll sizes of Bt cotton, Sikang 1 （small boll）, Sikang 3（middle boll）, Kemian 6（big boll）, were select- ed to study Bt endotoxin characteristics under source-sink regulation. The three cultivars were designed to be cut half leaves of the whole p!ar, t in 2005, and to be thinned half buds of the whole plant in 2006. The results showed that, with being cut half leaves of the wb-le plant, the boll volume, boll weight and seed weight decreased, and its effects on the boll volume and weight were greater in the big boll cultivar, reducing by 15.4% and 20.5% at 28 days after anthesis, respectively; in contrast, its effect on the seed weight was the biggest in the small boll cultivar, decreasing by 20.6% at 28 days after flowering. The Bt protein con- tents increased in the boll wall and seed, which increased by 23.5%~32.3%, 3.3%-18.8% at 21 days after anthesis, respectively. With half buds of the whole plant thinned, the boll volume, boll weight and seed weight were boosted, and the effects were the greatest in the big boll cultivar, increasing by 25.8%, 10.4%, 12.7% at 28 days after anthesis, respectively; in contrast, the Bt protein contents in the boll wall and seed decreased, the effects was greatest in the big boll cultivar too, reducing by 40.6%, 33.7% at 21 days after anthesis, respectively. There were significant negative correlations between the boll volume, boll weight and Bt endotoxin contents in the boll wall and seed under source-sink regulation, respectively, so was between seed weight and Bt endotoxin contents in the seed. The results suggest that the breeding and culture practice should aim at coordinating the rela- tion between the boll development and Bt endotoxin expression.

关 键 词：BT棉 源库调节 BT蛋白 铃体积 铃重 

分 类 号：S562[农业科学—作物学]