含酸性基团温度敏感色谱材料的制备及其在氨基酸分离中的应用  

Preparation of Temperature-Responsive Chromatographic Materials Containing Acidic Groups for Separation of Amino Acids

作　　者：刘宗建[1] 戴荣继[1] 梁笑[1] 武海燕[1] 顾昊[1] 丁薇[1] 钱庆庆[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命学院,北京100081

出　　处：《北京理工大学学报》2011年第9期1104-1108,共5页Transactions of Beijing Institute of Technology

基　　金：国家自然科学基金资助项目(20675008)

摘　　要：用自由基聚合法制备了分别含有聚(N-异丙基丙烯酰胺/2-丙烯酰胺基-2-甲基丙磺酸)和聚(异丙基丙烯酰胺/甲基丙烯酸异丁酯/丙烯酸)的2种酸性温敏色谱填料.用热失重和元素分析表征了接枝率.用HPLC研究了异硫氰酸苯酯衍生化丙氨酸、缬氨酸、苯丙氨酸、色氨酸、精氨酸、亮氨酸等6种氨基酸的色谱保留行为.结果显示,采用纯水相为流动相,仅通过控制柱温即可实现分离,证明被分离物的色谱保留性质随温度的改变而变化,制得的色谱填料具有温度敏感性.Chromatographic packing materials containing poly （N-isopropylacrylamide-co-2-aer- ylamido-2-methyl propane sulfonic acid） and poly（N-isopropylacrylamide-co-N-tert-butylacryla- mide） were prepared via conventional radical polymerization. Characterization was performed with thermogravimetrie analysis （TGA） and elemental analysis to determine the grafting ratio. Mixtures of Val, Leu, Phe, Trp, Art and Ala derivatized with phenylisothiocyanate were separated using prepared stationary phases by HPLC. The results indicated that separation can be accomplished only by controlling column temperatures, which proves that chromatographic retention of analytes alters with the change of column temperature, and the materials are of temperature-responsive property.

关 键 词：温度敏感聚合物 聚(N-异丙基丙烯酰胺) 色谱分离 

分 类 号：Q819[生物学—生物工程]