Burkholderia Vietnamiensis对水中结晶紫的降解  

THE BIODEGRADATION OF CRYSTAL VIOLET BY BURKHOLDERIA VIETNAMIENSIS

作　　者：程迎[1] 甘莉[1] 陈祖亮[1] 

机构地区：[1]福建师范大学化学与材料学院,福建福州350007

出　　处：《水处理技术》2011年第10期46-49,59,共5页Technology of Water Treatment

基　　金：福建省发改委项目基金(UE0021);福建师范大学闽江学者人才引进基金(200604)

摘　　要：从福州某印染厂活性污泥中分离筛选出1株对结晶紫染料有强脱色能力的菌株,根据其形态学特征和生理生化鉴定以及16S rRNA基因序列分析,鉴定为Burkholderia Vietnamiensis C09V。在振荡培养条件下对该菌株的脱色降解特性进行了研究,结果表明,菌株C09V的最佳碳源为葡萄糖;最佳氮源为KNO3;最适脱色初始pH为5.0;最佳菌投加体积分数为5%;最适脱色温度为35℃;在最适脱色条件下脱色36 h,该菌株对30 mg.L-1结晶紫脱色率可达到96.5%。A crystal violet-degrading bacterium, designed strain C09V was isolated from the activated sludge of the effluent treatment system （a printing and dyeing mill, fujian, china）, it was a subspecies of Burkholderia Vietnamiensis based on Gram staining, morphology characters, and biochemical tests, as well as the analysis of 16S rRNA gene similarities. Batch experiments were performed under various conditions, and the data showed that 96.5% of crystal violet could be degraded in the solution containing 30 mg. L^-1 crystal violet tested at the optima within 36 h： including glucose, KNO3, 5% inoculums size, pH at 5-0, and the temperature of 35 ℃.

关 键 词：BURKHOLDERIA Vietnamiensis 结晶紫 生物降解 脱色 

分 类 号：X703.1[环境科学与工程—环境工程]