双歧杆菌对溃疡性结肠炎小鼠外周血和肠系膜淋巴结CD4^+ CD25^+ Foxp3^+调节T细胞表达的影响  

The effect of Bifidobacterium on CD4^+ CD25^+ Foxp3^+ Treg of blood and lymphonodi mesenterici in mice with ulcerative colitis

作　　者：姚君[1] 王立生[1] 师瑞月[1] 王建尧[2] 李迎雪[1] 张茹[1] 莫镜[3] 

机构地区：[1]暨南大学第二临床医学院消化内科,广东深圳518020 [2]深圳市儿童医院,广东深圳518020 [3]广州医学院第三附属医院,广东广州510150

出　　处：《中国微生态学杂志》2011年第9期784-787,共4页Chinese Journal of Microecology

基　　金：广东省自然科学基金项目(10151802001000002)

摘　　要：目的探讨双歧杆菌治疗溃疡性结肠炎与CD4+ CD25+ Foxp3+调节T细胞的相关可能机制。方法采用DSS制作UC小鼠结肠炎模型,随机分成3组:正常对照(NC)组,模型(MD)组,双歧杆菌治疗(BbT)组。造模7 d后,给予双歧杆菌后续治疗7 d。评估小鼠疾病活动指数(DAI),结肠行HE染色及病理学评分(HDS);流式细胞仪检测外周血和肠系膜淋巴细胞中表达CD4+ CD25+ Foxp3+的Treg细胞的百分比率。结果 BbT组的DAI明显低于模型组(P<0.05);MD组HDS明显高于正常组(P<0.05);BbT组的HDS明显低于模型组(P<0.05);模型组外周血和肠系膜淋巴细胞CD4+ CD25+ Foxp3+Treg细胞占CD4+T细胞的百分率明显低于正常组(P<0.05);BbT组结肠外周血和肠系膜淋巴细胞CD4+ CD25+ Foxp3+Treg细胞占CD4+T细胞的百分率明显高于模型组(P<0.05)。结论双歧杆菌可以提高CD4+ CD25+ Foxp3+Treg数量,调节机体和肠道免疫功能,对UC发挥了一定的治疗作用。Objective To investigate the mechanism of Bifidobacteria treating the UC model mice through affecting the CD4＋CD25＋Foxp3＋ regulatory T cells.Method Ulcerative colitis was induced in mice by oral administration of synthetic dextran sulfate sodium for 7 days,then followed with normal drinking water or Bifidobacterium for an additional 7 days.The mice were divided into normal control（NC） group,colitis model（MD） group,and the Bifidobacterium treated（BbT） group.The Treg percentages of CD4＋CD25＋Foxp3＋/CD4＋ were detected with flow cytometry.Result The DAI were increased significantly in MD group compared with NC group,and decreased in the BbT group than in MD group.The HDS were increased significantly in MD group compared with NC group,and decreased in the BbT group than in MD group.The blood and lymphonodi mesenterici of the CD4＋CD25＋Foxp3＋/CD4＋ Treg percentages were decreased significantly in MD group compared with NC group（P0.05）,and increased in the BbT group than in MD group.Conclusion Bifidobacteria may play an important role in inhibiting the colonic inflammation in UC,by up-regulating CD4＋ CD25＋ Foxp3＋ Treg expression.

关 键 词：溃疡性结肠炎 双歧杆菌 CD4+ CD25+ Foxp3+调节T细胞 

分 类 号：R378.992[医药卫生—病原生物学]