微流控芯片药物诱导细胞凋亡  

Analysis of pharmaceutical-induced cell apoptosis by microfluidic chip

作　　者：汪耀[1] 李颖[1] 徐波[1] 冯晓均[1] 刘笔锋[1] 

机构地区：[1]华中科技大学生命科学与技术学院系统生物学系Britton Chance生物医学光子学研究中心,武汉430074

出　　处：《中国科学：化学》2011年第10期1629-1635,共7页SCIENTIA SINICA Chimica

基　　金：国家自然科学基金(30970692,20875035&30800286);国家重点基础研究发展计划(2007CB704507&2007CB914203)资助

摘　　要：发展了一种以微流控芯片为平台的药物诱导细胞凋亡的新方法.选择HeLa细胞为对象,通过浓度梯度芯片形成稳定的药物浓度梯度,诱导HeLa细胞凋亡,利用荧光能量共振转移(fluorescence resonance energy transfer,FRET)成像系统进行实时监测,分析细胞对不同浓度化合物的毒性反应.结果表明,细胞在顺铂诱导下发生明显的起泡和皱缩,FRET比率值逐渐降低,在药物浓度梯度作用下,芯片每个通道内细胞呈现不同程度的凋亡.该方法实现了药物浓度梯度诱导细胞凋亡的实时监测和定量分析,为抗肿瘤药物评价和高通量药物筛选提供了新的手段.Investigation on cell apoptosis is an important method for antitumor drug screening at the cellular level.This paper presented a microfluidic chip in which a stable drug concentration gradient of cisplatin was generated to induce the apoptosis of Hela cells.Cell apoptosis could be monitored in real-time through fluorescence resonance energy transfer（FRET） imaging system.The results showed that the cells bubbled and shrinked in morphology when the apoptosis happened,accompanied by gradually reduction of FRET signals.The degree of apoptosis proved to be drug concentration dependent on quantitation.This method provided a new way for anticancer drug evaluation at cellular level,with obvious advantages of high throughput,low cost and automation capability.

关 键 词：微流控芯片 细胞凋亡 抗肿瘤药物筛选 浓度梯度 

分 类 号：R96[医药卫生—药理学]