醇提三七总皂苷副产物中三七多糖的提取及含量测定  

Extraction and determination of polysaccharide in Panax notoginseng

作　　者：赵嵩月[1] 陈彤[1] 张要武 甘献文 吴廷丽 肖娜 杨如萍[1] 郭燕 

机构地区：[1]昆明医学院药学院,云南昆明650500 [2]云南云科药业有限责任公司,云南昆明650033

出　　处：《华西药学杂志》2011年第5期481-483,共3页West China Journal of Pharmaceutical Sciences

基　　金："十一五"国家科技支撑计划项目(课题任务书编号:2006BAI06A14-11);云南省重点产业创新工程(项目编号:2008IF022)

摘　　要：目的建立醇提三七总皂苷副产物三七多糖的提取及含量测定方法。方法分别收集生产上的三七废渣、大孔树脂水洗废液,浓缩,采用水提醇沉的提取工艺,提取三七多糖。用硫酸-蒽酮显色法测定多糖含量。结果三七废渣中提取物的收率为1.71%,多糖含量为56.70%;废液中提取物的收率为0.45%,含量为21.08%。测定三七多糖含量时,对照品在0.04～0.2 mg.mL-1的线性关系良好(r=0.9993),平均回收率为99.00%,RSD=3.18%(n=9)。结论从醇提皂苷的废渣、废液中提取多糖有应用价值;硫酸-蒽酮显色法测定三七多糖的含量,结果准确,操作简便。OBJECTIVE To establish a method for the extraction and determination of the polysaccharide from Panax notoginseng.METHODS The waste residue after extraction of the total saponins form Panax notoginseng was extracted with water-decoction alcohol-precipitation method.The waste liquor was condensed and extracted with alcohol-precipitation method,and Anthrone test was employed to determine the content of Panax notoginseng polysaccharide.RESULTS The linearity of glucose reference substance was good in the range of 0.04-0.2 mg·mL-1（r=0.9993）,and the average recovery was 99.00% with RSD of 3.18%（n=9）.The yield of Panax notoginseng polysaccharide in waste residue was 1.71%（purity 56.70%） and in waste liquor was 0.45%（purity 21.08%）.CONCLUSION The extraction technology from the waste residue and liquor after extraction of the total saponins of Panax notoginseng is valuable.Anthrone test is accurate,rapid and convenient and can be used for the determination of Panax notoginseng polysaccharide.

关 键 词：三七 三七总皂苷 三七多糖 硫酸-蒽酮法 综合利用 

分 类 号：R284[医药卫生—中药学]