HPLC同时测定兔血浆中的丙泊酚及其前药HX0969w  

Simultaneous determination of Propofol and its prodrug HX0969w in rabbit plasma by HPLC

作　　者：王瑜[1,2] 殷望[1,2] 张文胜[2] 杨俊[2] 康仪[2] 

机构地区：[1]四川大学华西药学院,四川成都610041 [2]四川大学华西医院麻醉与危重急救研究室,四川成都610041

出　　处：《华西药学杂志》2011年第5期490-492,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC法同时测定兔血浆中的丙泊酚及其前药HX0969w。方法采用Agilent Eclipse-Plus C18色谱柱(150mm×4.6 mm,5μm);流动相为甲醇-水-10%四丁基氢氧化铵(70∶29∶1,加入0.1%三乙胺,磷酸调pH7),流速1 mL.min-1;HX0969w的检测波长为260 nm,丙泊酚为272 nm。结果 HX0969w与丙泊酚的线性范围分别为46.5～2973.6μg.mL-1(r=0.9997)、9.4～601.0μg.mL-1(r=0.9993);平均回收率分别为95.97%、98.08%,日内RSD≤4.22%,日间RSD≤7.92%(n=5)。结论新建方法可简便、准确、迅速地同时测定血浆中前药HX0969w和丙泊酚的含量,可用于HX0969w药物动力学的初步研究和原料与制剂中前药和丙泊酚的含量测定。OBJECTIVE To establish an HPLC method for simultaneously determining the concentration of Propofol and its prodrug HX0969w in rabbit plasma.METHODS HPLC method was adopted.An Agilent eclipse plus C18 column（150 mm×4.6 mm,5 μm） was used.The mobile phase consisted of methanol-water-10% tetrabutyl ammonium hydroxide（70：29：1,with 0.1% triethylamine,adding phosphoric acid to adjust pH7.0）.The flow rate was 1 mL·min-1,the detection wavelength was 260 nm for HX0969w and 272 nm for propofol.RESULTS The linear range of HX0969w was 46.5-2973.6 μg·mL-1（r=0.9997）,the average recovery was 95.97 %.The linear range of propofol was 9.4-601.0 μg·mL-1（r=0.9993）,the average recovery was 98.08%.The intra and inter-assay precision for this analysis were less than 4.22% and 7.92%,respectively.CONCLUSION The method is simple,accurate,rapid,and can be used for the simultaneous determination of propofol and its prodrug HX0969w in plasma.Thus the method is suitable for pharmacokinetic studies of HX0969w and the content detection of the raw material or preparation.

关 键 词：前药 HX0969w 丙泊酚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]